Novel haloalkaliphilic methanotrophic bacteria: An attempt for enhancing methane bio-refinery
| Autor: | Alfons J. M. Stams, Lidia J. Sadornil, Pedro A. García-Encina, Raúl Muñoz, Sara Cantera, Irene Sánchez-Andrea |
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| Rok vydání: | 2019 |
| Předmět: |
Environmental Engineering
Bioconversion 0208 environmental biotechnology Alishewanella 02 engineering and technology 010501 environmental sciences Management Monitoring Policy and Law Ectoine Microbiology 01 natural sciences Methane chemistry.chemical_compound Microbiologie RNA Ribosomal 16S Biomass Food science Waste Management and Disposal 0105 earth and related environmental sciences Halomonas WIMEK Bacteria biology Chemistry MicPhys General Medicine 16S ribosomal RNA biology.organism_classification Methane treatment Metano Carbon 020801 environmental engineering CH bio-refinery Energy source |
| Zdroj: | Journal of Environmental Management, 231, 1091-1099 UVaDOC. Repositorio Documental de la Universidad de Valladolid instname Journal of Environmental Management 231 (2019) |
| ISSN: | 0301-4797 |
| DOI: | 10.1016/j.jenvman.2018.11.017 |
| Popis: | Producción Científica Methane bioconversion into products with a high market value, such as ectoine or hydroxyectoine, can be optimized via isolation of more efficient novel methanotrophic bacteria. The research here presented focused on the enrichment of methanotrophic consortia able to co-produce different ectoines during CH4 metabolism. Four different enrichments (Cow3, Slu3, Cow6 and Slu6) were carried out in basal media supplemented with 3 and 6% NaCl, and using methane as the sole carbon and energy source. The highest ectoine accumulation (∼20 mg ectoine g biomass−1) was recorded in the two consortia enriched at 6% NaCl (Cow6 and Slu6). Moreover, hydroxyectoine was detected for the first time using methane as a feedstock in Cow6 and Slu6 (∼5 mg g biomass−1). The majority of the haloalkaliphilic bacteria identified by 16S rRNA community profiling in both consortia have not been previously described as methanotrophs. From these enrichments, two novel strains (representing novel species) capable of using methane as the sole carbon and energy source were isolated: Alishewanella sp. strain RM1 and Halomonas sp. strain PGE1. Halomonas sp. strain PGE1 showed higher ectoine yields (70–92 mg ectoine g biomass−1) than those previously described for other methanotrophs under continuous cultivation mode (∼37–70 mg ectoine g biomass−1). The results here obtained highlight the potential of isolating novel methanotrophs in order to boost the competitiveness of industrial CH4-based ectoine production. Ministerio de Economía, Industria y Competitividad (Project CTM2015-70442-R project and Red NOVEDAR) Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. UIC71) European Union through the FEDER Funding Program (PhD Grant contract Nº E-47-2014-0140696) |
| Databáze: | OpenAIRE |
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