Organic solutes in the deepest phylogenetic branches of the Bacteria: identification of α(1–6)glucosyl-α(1–2)glucosylglycerate in Persephonella marina
| Autor: | Pedro Lamosa, Rita Ventura, Christopher D. Maycock, Helena Santos, Neil D.H. Raven, Marta V. Rodrigues, Luís G. Gonçalves, Jean Carr |
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| Rok vydání: | 2012 |
| Předmět: |
Hot Temperature
ved/biology.organism_classification_rank.species Sodium Chloride Marinitoga piezophila Glyceric Acids Microbiology 03 medical and health sciences Protein structure 030304 developmental biology Alanine chemistry.chemical_classification 0303 health sciences Bacteria biology 030306 microbiology ved/biology Thermophile General Medicine biology.organism_classification Adaptation Physiological Persephonella marina Amino acid Biochemistry chemistry Molecular Medicine Osmoprotectant |
| Zdroj: | Extremophiles. 17:137-146 |
| ISSN: | 1433-4909 1431-0651 |
| Popis: | The accumulation of organic solutes was investigated in the thermophilic bacteria Persephonella marina and Marinitoga piezophila, two representatives of the deepest lineages in the domain Bacteria. These organisms grow optimally at around 70 °C in medium containing 3 % NaCl. A new disaccharide, accumulating in Persephonella marina, was identified as α(1-6)glucosyl-α(1-2)glucosylglycerate (GGG), by nuclear magnetic resonance. This identification was validated by comparison with the spectra of the compound obtained by chemical synthesis. Besides GGG, the solute pool of Persephonella marina comprised β-glutamate, di-myo-inositol-1,3'-phosphate and 2-O-α-glucosylglycerate. In contrast, amino acids such as α-glutamate, proline and alanine were the dominant components of the solute pool of Marinitoga piezophila and sugar derivatives were absent. The ability of GGG to protect protein structure against heat denaturation was assessed using model proteins. A genomic search for the biosynthetic pathways of known ionic solutes in Aquificales and Thermotogales shows the inability of this analysis to predict the nature of compatible solutes and underlines the need for efficient cultivation techniques. |
| Databáze: | OpenAIRE |
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