A non-essential glutamyl aminopeptidase is required for optimal growth of Lactococcus lactis MG1363 in milk
| Autor: | H. G. Griffin, Michael J. Gasson, F. Mulholland, S. Movahedi, K. J. A. L'anson |
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| Rok vydání: | 1995 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data medicine.disease_cause Glutamyl Aminopeptidase Microbiology Aminopeptidases Open Reading Frames Cellulase medicine Escherichia coli Animals Genomic library Amino Acid Sequence Cloning Molecular Peptide sequence DNA Primers Gene Library Clostridium PEPA biology Base Sequence Sequence Homology Amino Acid Lactococcus lactis Nucleic acid sequence biology.organism_classification Open reading frame Milk Biochemistry Mutagenesis Glutamyl aminopeptidase |
| Zdroj: | Microbiology (Reading, England). 141 |
| ISSN: | 1350-0872 |
| Popis: | Degenerate PCR primers were designed from the N-terminal amino acid sequence of a glutamyl aminopeptidase (PepA) from Lactococcus lactis. These primers were used to screen a lambda library for clones containing the gene (pepA) encoding PepA. The DNA sequence of a 2.1 kb fragment containing pepA was determined. The sequence revealed the presence of one complete and two incomplete open reading frames (ORFs). The complete ORF encodes a putative protein of 353 amino acids with a predicted N-terminal sequence identical to that determined for purified PepA. The pepA gene was subcloned on an Escherichia coli plasmid vector and production of active PepA was confirmed by means of a zymogram. Mutants of L. lactis in which the pepA gene was inactivated grew to normal cell densities in milk but exhibited a reduced growth rate during the exponential phase. Thus whilst PepA is required for optimal growth it is not essential. |
| Databáze: | OpenAIRE |
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