Role of platelet-derived growth factor in development of in vitro matured and in vitro fertilized bovine embryos
| Autor: | J. K. Thibodeaux, W. Hansel, R. P. Del Vecchio |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Embryology
medicine.medical_specialty Fertilization in Vitro Andrology Embryonic and Fetal Development Endocrinology Internal medicine medicine Animals Inner cell mass Blastocyst Bovine serum albumin Cells Cultured Fallopian Tubes Platelet-Derived Growth Factor biology Embryogenesis Obstetrics and Gynecology Embryo Cell Biology Embryo Mammalian In vitro maturation medicine.anatomical_structure Reproductive Medicine embryonic structures biology.protein Oviduct Cattle Female Platelet-derived growth factor receptor |
| Zdroj: | Reproduction. 98:61-66 |
| ISSN: | 1741-7899 1470-1626 |
| Popis: | This experiment was designed to determine whether the stimulatory effects of bovine oviductal epithelial cells (BOEC) on development of early bovine embryos are due to platelet-derived growth factor (PDGF). Four hundred and twenty five 8-cell bovine embryos derived from in vitro maturation and in vitro fertilization procedures were equally and randomly allotted to one of the following culture treatment groups: control medium alone (Menezo's B2 medium; MB2), MB2 with 1 ng PDGF ml-1 (PDGF), 1 ng PDGF ml-1 and 10 micrograms anti-PDGF antibody ml-1 (PDGF + Ab), BOEC or BOEC and 10 micrograms anti-PDGF antibody ml-1 (BOEC + Ab). All embryos were cultured in 100 microliters of serum-free MB2 medium supplemented with 2 mg fatty-acid-free bovine serum albumin ml-1. Embryos for all treatment groups were incubated at 39 degrees C and 5% CO2 in humidified air in groups of five embryos per well in 96-well culture plates until 7 days after in vitro insemination. A higher proportion of embryos developed to > 8-cell and to the morula stage following culture with PDGF, BOEC or BOEC+Ab than with MB2 alone. Incubation of PDGF and BOEC-treated embryos with anti-PDGF reduced development to the morula and blastocyst stages. However, anti-PDGF did not completely inhibit blastocyst development when added to BOEC. In addition, embryos incubated with BOEC and anti-PDGF contained a reduced number of inner cell mass cells compared with embryos incubated with BOEC alone. These results indicate that PDGF provides a developmental stimulus similar to BOEC for bovine embryos at the fourth cell cycle.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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