MicroRNAs transfer from human macrophages to hepato-carcinoma cells and inhibit proliferation
| Autor: | Anne Aucher, Daniel M. Davis, Dominika Rudnicka |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cell signaling
Carcinoma Hepatocellular Recombinant Fusion Proteins Immunology Cell Communication Biology Exosomes Transfection Article Receptor IGF Type 1 Cell-Derived Microparticles Genes Reporter Cell Line Tumor microRNA Immunology and Allergy Humans RNA Small Interfering Cytoskeleton Cell Line Transformed Regulation of gene expression Macrophages Liver Neoplasms RNA Hep G2 Cells HCCS Mastocytoma Bridged Bicyclo Compounds Heterocyclic Microvesicles Coculture Techniques Cell biology Gene Expression Regulation Neoplastic MicroRNAs Cell culture Leukemia Monocytic Acute Stathmin Thiazolidines Cell Division |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 191(12) |
| ISSN: | 1550-6606 |
| Popis: | Recent research has indicated a new mode of intercellular communication facilitated by the movement of RNA between cells. There is evidence that RNA can transfer between cells in a multitude of ways, including in complex with proteins or lipids or in vesicles, including apoptotic bodies and exosomes. However, there remains little understanding of the function of nucleic acid transfer between human cells. In this article, we report that human macrophages transfer microRNAs (miRNAs) to hepato-carcinoma cells (HCCs) in a manner that required intercellular contact and involved gap junctions. Two specific miRNAs transferred efficiently between these cells—miR-142 and miR-223—and both were endogenously expressed in macrophages and not in HCCs. Transfer of these miRNAs influenced posttranscriptional regulation of proteins in HCCs, including decreased expression of reporter proteins and endogenously expressed stathmin-1 and insulin-like growth factor-1 receptor. Importantly, transfer of miRNAs from macrophages functionally inhibited proliferation of these cancerous cells. Thus, these data led us to propose that intercellular transfer of miRNA from immune cells could serve as a new defense against unwanted cell proliferation or tumor growth. |
| Databáze: | OpenAIRE |
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