The interplay between iron limitation, light, and carbon in the proteorhodopsin containing Photobacterium angustum S14
| Autor: | Coco Koedooder, Ingrid Obernosterer, Rémy Van Geersdaële, Audrey Guéneuguès, Stéphane Blain, François-Yves Bouget |
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| Přispěvatelé: | Laboratoire d'Océanographie Microbienne (LOMIC), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Rhodopsin proteorhodopsin Iron 030106 microbiology Applied Microbiology and Biotechnology Microbiology Photoheterotroph Respiratory electron transport chain 03 medical and health sciences Gene expression Rhodopsins Microbial [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography Regulation of gene expression Photobacterium angustum S14 Proteorhodopsin Ecology Phototroph biology Photobacterium Photoheterotrophy Fe-limitation biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Carbon 030104 developmental biology 13. Climate action biology.protein Biophysics [SDE.BE]Environmental Sciences/Biodiversity and Ecology Intracellular |
| Zdroj: | FEMS Microbiology Letters FEMS Microbiology Letters, Wiley-Blackwell, 2020, ⟨10.1093/femsec/fiaa103⟩ |
| ISSN: | 0378-1097 1574-6968 |
| DOI: | 10.1093/femsec/fiaa103⟩ |
| Popis: | Iron (Fe) limitation is known to affect heterotrophic bacteria within the respiratory electron transport chain, therefore strongly impacting the overall intracellular energy production. We investigated whether the gene expression pattern of the light-sensitive proton pump, proteorhodopsin (PR), is influenced by varying light, carbon and Fe concentrations in the marine bacterium Photobacterium angustum S14 and whether PR can alleviate the physiological processes associated with Fe starvation. Our results show that the gene expression of PR increases as cells enter the stationary phase, irrespective of Fe-replete or Fe-limiting conditions. This upregulation is coupled to a reduction in cell size, indicating that PR gene regulation is associated with a specific starvation-stress response. We provide experimental evidence that PR gene expression does not result in an increased growth rate, cell abundance, enhanced survival or ATP concentration within the cell in either Fe-replete or Fe-limiting conditions. However, independent of PR gene expression, the presence of light did influence bacterial growth rates and maximum cell abundances under varying Fe regimes. Our observations support previous results indicating that PR phototrophy seems to play an important role within the stationary phase for several members of the Vibrionaceae family, but that the exact role of PR in Fe limitation remains to be further explored. |
| Databáze: | OpenAIRE |
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