Simultaneous determination of all-trans and 13-cis retinoic acids and their 4-oxo metabolites by adsorption liquid chromatography after solid-phase extraction
| Autor: | M Cornic, B Gourmel, A Agadir, C Dreux, Laurent Degos, Christine Chomienne, P Lefebvre, B Hue |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Acute promyelocytic leukemia
Detection limit Chromatography Metabolite Retinoic acid Reproducibility of Results Tretinoin General Chemistry medicine.disease High-performance liquid chromatography chemistry.chemical_compound Acetic acid Leukemia Promyelocytic Acute chemistry medicine Humans Spectrophotometry Ultraviolet Adsorption Solid phase extraction Isotretinoin Chromatography High Pressure Liquid Active metabolite |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 666:55-61 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(94)00554-i |
| Popis: | All- trans retinoic acid (all- trans RA), the active metabolite of vitamin A, has been demonstrated to be an efficient alternative to chemotherapy in the treatment of acute promyelocytic leukemia (APL), the AML 3 subtype of the FAB cytological classification. Complete remission is obtained by inducing terminal granulocytic differentiation of the leukemic cells. To study all- trans RA pharmacokinetics in patients with APL, a rapid, precise and selective high-performance liquid chromatographis (HPLC) assay was developed. This method is easy and shows good repeatability (C.V. = 8.41–12.44%), reproducibility (C.V. = 9.19–14.73%), accuracy (C.V. = 3.5–11%) and sensitivity with a detection limit of 5 pmol/ml. The analysis is performed using normal-phase HPLC in an isocratic mode with UV detection after solid-phase extraction on octadecyl (C 18 ) columns. The mobile phase is hexanedichloromethane-dioxane (78:18:4, v/v) containing 1% acetic acid. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|