Effects of Bisphosphonate Administration on Cleft Bone Graft in a Rat Model
| Autor: | Taewoo Kwon, Nicole Cheng, Jeffrey L. Olson, Deborah Lee, Sotirios Tetradis, Reuben Kim, Rachel Lim, Christine Hong, Sandy Ha, Kang Ting, Xinli Zhang, Juyoung Park |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty medicine.medical_treatment Rat model Zoledronic Acid Article Ilium 03 medical and health sciences Bone volume fraction 0302 clinical medicine critical-sized defect Alveolar Process medicine Animals Femur Dental/Oral and Craniofacial Disease Bone Resorption bisphosphonates Saline Inbred F344 Transplantation Bone Transplantation Diphosphonates biology business.industry Imidazoles Acid phosphatase X-Ray Microtomography 030206 dentistry Bisphosphonate Rats Inbred F344 Rats Resorption Surgery 030104 developmental biology Zoledronic acid Otorhinolaryngology Dentistry Systemic administration biology.protein Female Oral Surgery business cleft lip and palate medicine.drug |
| Zdroj: | The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association, vol 54, iss 6 |
| ISSN: | 1545-1569 1055-6656 |
| Popis: | ObjectiveBone grafts in patients with cleft lip and palate can undergo a significant amount of resorption. The aim of this study was to investigate the effects of bisphosphonates (BPs) on the success of bone grafts in rats.DesignThirty-five female 15-week-old Fischer F344 Inbred rats were divided into the following experimental groups, each receiving bone grafts to repair an intraoral CSD: (1) Graft/saline: systemic administration of saline and (2) systemic administration of zoledronic acid immediately following surgery (graft/BP/T0), (3) 1 week postoperatively (graft/BP/T1), and (4) 3 weeks postoperatively (graft/BP/T2). As an additional control, the defect was left empty without bone graft.Main Outcome MeasuresMicrocomputed tomography and histologic analyses were performed in addition to evaluation of osteoclasts through tartrate-resistant acid phosphatase staining.ResultsBone volume fraction (bone volume/tissue volume) for the delayed BP treatment groups (graft/BP/T1 = 45.4% ± 8.8%; graft/BP/T2 = 46.1% ± 12.4%) were significantly greater than that for the graft/saline group (31.0% ± 7.9%) and the graft/BP/T0 (27.6% ± 5.9%) 6 weeks postoperatively ( P < .05). Hematoxylin and eosin staining confirmed an evident increase in bone volume and fusion of defect margins with existing palatal bone in the graft/BP/T1 and graft/BP/T2 groups. The graft/BP/T0 group showed the lowest bone volume with signs of acute inflammation.ConclusionsDelayed BP administration following cleft bone graft surgery led to significant increase in bone volume and integration compared with saline controls. However, BP injection immediately after the surgery did not enhance bone volume, and rather, may negatively affect bone graft incorporation. |
| Databáze: | OpenAIRE |
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