A reagentless aptasensor based on intrinsic aptamer redox activity for the detection of tetracycline in water
| Autor: | Carole Calas-Blanchard, Georges Istamboulie, Thierry Noguer, Ahmad Alawad |
|---|---|
| Přispěvatelé: | IRCELYON-Analyse chimique, texture (ANALYSE), Institut de recherches sur la catalyse et l'environnement de Lyon (IRCELYON), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), PIERRE FABRE-EDF (EDF)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Tetracycline
Aptamer 02 engineering and technology 010402 general chemistry Electrochemistry 01 natural sciences Redox [SPI]Engineering Sciences [physics] Materials Chemistry medicine Electrical and Electronic Engineering Instrumentation Detection limit Oligonucleotide Chemistry Metals and Alloys 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Electrode Cyclic voltammetry 0210 nano-technology Nuclear chemistry medicine.drug |
| Zdroj: | Sensors and Actuators B: Chemical Sensors and Actuators B: Chemical, Elsevier, 2019, 288, pp.141-146. ⟨10.1016/j.snb.2019.02.103⟩ |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2019.02.103⟩ |
| Popis: | A label-free electrochemical aptasensor was fabricated for reagentless determination of tetracycline (TET) in water. A 5′-amino-modified 76-mer oligonucleotide was immobilized on screen-printed carbon electrode via carbodimide coupling. Cyclic voltammetry experiments revealed an unexpected electroactivity of immobilized aptamer, showing a quasi-reversible signal with oxidation and reduction peaks at respectively +0.19 and −0.25 V vs Ag/AgCl. The binding of TET on the aptasensor induced a decrease of oxidation peak, allowing the detection of TET for concentrations ranging from 0.05 μg/L to 20 μg/L, with a detection limit of 0.035 μg/L. The proposed aptasensor displayed a good specificity for TET and was successfully used for the detection of TET in natural waters, with recovery rates ranging from 87.8 to 97%. |
| Databáze: | OpenAIRE |
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