Bone marrow stromal cells can be delivered to the site of traumatic brain injury via intrathecal transplantation in rabbits
| Autor: | Xiao-dan Jiang, Ying-qian Cai, Xiafei Fu, Mouxuan Du, Shaojie Cui, Wen-peng Liu, Ru-xiang Xu |
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| Rok vydání: | 2008 |
| Předmět: |
Pathology
medicine.medical_specialty Stromal cell Traumatic brain injury Green Fluorescent Proteins Central nervous system Apoptosis Bone Marrow Cells Transfection Precursor cell Animals Autologous transplantation Medicine Injections Spinal Bone Marrow Transplantation medicine.diagnostic_test business.industry Lumbar puncture General Neuroscience Graft Survival Flow Cytometry medicine.disease Transplantation medicine.anatomical_structure Brain Injuries Rabbits Bone marrow Stromal Cells business |
| Zdroj: | Neuroscience Letters. 434:160-164 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/j.neulet.2007.12.067 |
| Popis: | Recent studies suggest that bone marrow stromal cells (BMSCs) are promising grafts for treatment of traumatic brain injury (TBI). Neural precursor cells (NPCs) have been detected in the site of cervical cord injury following intrathecal injection by lumbar puncture. So, this study is designed to determine whether BMSCs (after intrathecal administration by lumbar puncture) could also migrate to the TBI site. The cells were cultured in vitro and transfected with adenovirus green fluorescent protein (Ad-GFP), and then transplanted intrathecally or intravenously into an autologous rabbit model of TBI. The labeled, grafted cells were identified in the injured cerebral tissue using fluorescence microscopy. Results showed that the intrathecal protocol was more efficient than the intravenous one. And motor dysfunction was improved after autologous transplantation of BMSCs. This study suggests another attractive minimally invasive option for treating TBI. |
| Databáze: | OpenAIRE |
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