Characterization of Momordica charantia L. polysaccharide and its protective effect on pancreatic cells injury in STZ-induced diabetic mice
| Autor: | Hongman Chen, Cong Zhang, Weiqi Bai |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
Momordica charantia endocrine system diseases Serum insulin 02 engineering and technology Pharmacology Polysaccharide 01 natural sciences Biochemistry Diabetes Mellitus Experimental Mice chemistry.chemical_compound Polysaccharides Structural Biology Diabetes mellitus medicine Animals Sugar Pancreas Molecular Biology chemistry.chemical_classification Momordica biology 010405 organic chemistry Chemistry Diabetic mouse General Medicine 021001 nanoscience & nanotechnology medicine.disease Streptozotocin biology.organism_classification Glucuronic acid 0104 chemical sciences Molecular Weight Cytoprotection 0210 nano-technology medicine.drug |
| Zdroj: | International Journal of Biological Macromolecules. 115:45-52 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2018.04.039 |
| Popis: | A polysaccharide with a molecular weight of 13,029Da was isolated from Momordica charantia (MCP) fruit and purified by ion-exchange and size-exclusion chromatography. The isolated polysaccharide MCPIIa contained L-Rha, D-GalA, D-Gal, D-Xyl, L-Ara in a molar ratio of 12:3.05:19.89:5.95:56. IR spectrum and NMR studies indicated that the MCPIIa sugar units were linked, via β-glycosidic bonds, to a large number of arabinofuranose, glucuronic acid, and xylopyranosyl residues. In addition, the hypoglycemic effect of MCPIIa was investigated in streptozotocin (STZ)-induced diabetic mice. After STZ-induction, MCPIIa (100, 200, or 300mg/kg body weight) was administered orally, once daily, for 28days. Glycemia in STZ-diabetogenic mice was significantly reduced, and compared with diabetes mellitus (DM) mice, serum insulin concentration increased significantly, following MCPIIa administration. Transmission electron microscopy showed an alleviation of STZ-lesions in pancreatic tissue from mice treated with MCPIIa. These results indicate that MCPIIa may be useful as an anti-diabetic agent. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect