Betaine homocysteine methyltransferase is active in the mouse blastocyst and promotes inner cell mass development
| Autor: | Jacquetta M. Trasler, Michael Lever, Sandy Slow, Baohua Zhang, Jay M. Baltz, Megan Kooistra, Amanda L. Fortier, Martin B. Lee, Timothy A. Garrow |
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| Rok vydání: | 2012 |
| Předmět: |
S-Adenosylmethionine
Methyltransferase animal structures Cell Survival Embryonic Development Biology Biochemistry Morula chemistry.chemical_compound Mice Betaine medicine Inner cell mass Animals Blastocyst RNA Messenger Molecular Biology reproductive and urinary physiology Gene knockdown Methionine urogenital system Embryo Cell Biology Methylation Cell biology medicine.anatomical_structure chemistry Betaine-Homocysteine S-Methyltransferase Gene Knockdown Techniques embryonic structures Female Developmental Biology |
| Zdroj: | The Journal of biological chemistry. 287(39) |
| ISSN: | 1083-351X |
| Popis: | Methyltransferases are an important group of enzymes with diverse roles that include epigenetic gene regulation. The universal donor of methyl groups for methyltransferases is S-adenosylmethionine (AdoMet), which in most cells is synthesized using methyl groups carried by a derivative of folic acid. Another mechanism for AdoMet synthesis uses betaine as the methyl donor via the enzyme betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5), but it has been considered to be significant only in liver. Here, we show that mouse preimplantation embryos contain endogenous betaine; Bhmt mRNA is first expressed at the morula stage; BHMT is abundant at the blastocyst stage but not other preimplantation stages, and BHMT activity is similarly detectable in blastocyst homogenates but not those of two-cell or morula stage embryos. Knockdown of BHMT protein levels and reduction of enzyme activity using Bhmt-specific antisense morpholinos or a selective BHMT inhibitor resulted in decreased development of embryos to the blastocyst stage in vitro and a reduction in inner cell mass cell number in blastocysts. The detrimental effects of BHMT knockdown were fully rescued by the immediate methyl-carrying product of BHMT, methionine. A physiological role for betaine and BHMT in blastocyst viability was further indicated by increased fetal resorption following embryo transfer of BHMT knockdown blastocysts versus control. Thus, mouse blastocysts are unusual in being able to generate AdoMet not only by the ubiquitous folate-dependent mechanism but also from betaine metabolized by BHMT, likely a significant pool of methyl groups in blastocysts. |
| Databáze: | OpenAIRE |
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