A baboon model for hematologic studies of cardiopulmonary bypass
Autor: | Ling Sun, C.Erik Hack, Henk te Velthuis, Nicolas Gikakis, Cezary Marcinkiewicz, Mohammad M.H Khan, A. Koneti Rao, Yuji Hiramatsu, L. Henry Edmunds, Robert W. Colman, Harry L. Anderson, Joseph H. Gorman, S Niewiarowski |
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Přispěvatelé: | Faculteit der Geneeskunde, VU University medical center |
Rok vydání: | 1997 |
Předmět: |
Blood Platelets
Membrane oxygenator Blood Pressure Oxygenators Pharmacology Neutrophil Activation Pathology and Forensic Medicine law.invention Bubble oxygenator Heart Rate law biology.animal Leukocytes Cardiopulmonary bypass Animals Medicine Platelet Cardiac Output Complement Activation Oxygenator Platelet-poor plasma Cardiopulmonary Bypass biology business.industry Models Cardiovascular Thrombosis Blood Proteins General Medicine Blood Physiological Phenomena Platelet Activation Blood proteins Adenosine Diphosphate surgical procedures operative Immunology Female business Biomarkers Papio circulatory and respiratory physiology Baboon |
Zdroj: | J LAB CLIN MED, 130, 412-420. Mosby Inc. The Journal of Laboratory and Clinical Medicine, 130, 412-420. Mosby Inc. Hiramatsu, Y, Gigakis, N, Gorman, J H, Khan, M M H, Hack, C E, te Velthuis, H, Sun, L, Marcinkiewicz, C, Rao, A K, Niewiarowski, S, Colman, R W & Edmunds, L H 1997, ' A baboon model for hematologic studies of cardiopulmonary bypass ', The Journal of Laboratory and Clinical Medicine, vol. 130, pp. 412-420 . https://doi.org/10.1016/S0022-2143(97)90041-X |
ISSN: | 0022-2143 |
Popis: | Objective investigation of new inhibitors of blood protein or cellular systems that are activated during cardiopulmonary bypass (CPB) is impeded by the absence of a satisfactory animal model. Because most baboon hematologic proteins immunologically cross-react with those used for human assays, we developed a robust, reusable baboon model of CPB. Blood samples were obtained from adult baboons at six time intervals before, during, and after 60 minutes of partial CPB at 37 ° C with peripheral cannulas. Both membrane (n = 7) and bubble oxygenators (n = 7) were investigated. We measured platelet and white blood cell counts; platelet response to adenosine diphosphate and release of l~-thromboglobulin; fibrinopeptide A, prothrombin fragment FI.2, thrombin-antithrombin complex, D-dimer, and plasminantiplasmin complex; activated complement (C3b/c and C4b/c); elastase-=1 proteinase inhibitor complex; and bleeding times. Adherent glycoprotein IIio antigen in Triton X- 100 washes of the perfusion circuit was also measured. Markers of baboon platelet, complement, and neutrophil activation and thrombosis significantly increased during CPB with bubble oxygenator systems but did not change appreciably in membrane oxygenator circuits. Markers of fibrinolysis, o-dimer, and plasmin-antiplasmin complex did not change with either oxygenator. The baboon model of CPB, when a bubble oxygenator is used, is a robust, reusable animal model for evaluating inhibitors of platelet, complement, and neutrophil activation and thrombosis during and after CPB. (J Lab Clin Med 1997;130:412-20) |
Databáze: | OpenAIRE |
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