High positive predictive value of Gram stain on catheter-drawn blood samples for the diagnosis of catheter-related bloodstream infection in intensive care neonates
Autor: | Danièle Vermeylen, B. van Overmeire, Yves Hennequin, M. Deleers, Sandrine Roisin, Magali Dodémont, Olivier Denis |
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Rok vydání: | 2015 |
Předmět: |
0301 basic medicine
Microbiology (medical) Microbiological Techniques medicine.medical_specialty Neonatal intensive care unit 030106 microbiology Sensitivity and Specificity law.invention Sepsis Hospitals University 03 medical and health sciences 0302 clinical medicine Medical microbiology Belgium law Predictive Value of Tests Intensive care Internal medicine Intensive Care Units Neonatal medicine Humans Blood culture 030212 general & internal medicine Retrospective Studies medicine.diagnostic_test Staining and Labeling business.industry Infant Newborn General Medicine medicine.disease Surgery Catheter Infectious Diseases Gram staining Blood Predictive value of tests Catheter-Related Infections Phenazines Gentian Violet business |
Zdroj: | European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology. 35(4) |
ISSN: | 1435-4373 |
Popis: | Catheter-related bloodstream infections (CRBSIs) remain a leading cause of healthcare-associated infections in preterm infants. Rapid and accurate methods for the diagnosis of CRBSIs are needed in order to implement timely and appropriate treatment. A retrospective study was conducted during a 7-year period (2005-2012) in the neonatal intensive care unit of the University Hospital Erasme to assess the value of Gram stain on catheter-drawn blood samples (CDBS) to predict CRBSIs. Both peripheral samples and CDBS were obtained from neonates with clinically suspected CRBSI. Gram stain, automated culture and quantitative cultures on blood agar plates were performed for each sample. The paired quantitative blood culture was used as the standard to define CRBSI. Out of 397 episodes of suspected CRBSIs, 35 were confirmed by a positive ratio of quantitative culture (>5) or a colony count of CDBS culture >100 colony-forming units (CFU)/mL. All but two of the 30 patients who had a CDBS with a positive Gram stain were confirmed as having a CRBSI. Seven patients who had a CDBS with a negative Gram stain were diagnosed as CRBSI. The sensitivity, specificity, positive predictive value and negative predictive value of Gram stain on CDBS were 80, 99.4, 93.3 and 98.1 %, respectively. Gram staining on CDBS is a viable method for rapidly ( |
Databáze: | OpenAIRE |
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