Luminescence lifetime imaging of ultra-long room temperature phosphorescence on a smartphone
Autor: | Di Tian, Teng Ma, Zece Zhu, Ye Sun, Jintao Zhu |
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Rok vydání: | 2021 |
Předmět: |
Luminescence
Materials science Mice Nude 02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry law.invention Mice Persistent luminescence law Animals Skin Mice Inbred BALB C Millisecond Pixel business.industry 010401 analytical chemistry Temperature 021001 nanoscience & nanotechnology 0104 chemical sciences Lens (optics) Dichroic filter Optoelectronics Female Synchronous control Smartphone 0210 nano-technology business Phosphorescence |
Zdroj: | Analytical and Bioanalytical Chemistry. 413:3291-3297 |
ISSN: | 1618-2650 1618-2642 |
DOI: | 10.1007/s00216-021-03266-y |
Popis: | Luminescence lifetime imaging plays an important role in distinguishing the luminescence decay rates in time-resolved luminescence imaging. However, traditional imaging instruments used for detecting lifetimes within milliseconds would be time-consuming when imaging ultra-long luminescence lifetimes over subseconds. Herein, we present an accessible and simple optical system for detecting lifetimes of persistent luminescence. A smartphone integrated with a UV LED, a dichroic mirror, and a lens was used for recording the persistent luminescence. With only a few seconds of data acquisition, a luminescence lifetime image could be processed from the video by exponential fitting of the gray level of each pixel to the delay time. Since this approach only requires single excitation, no synchronous control is needed, greatly simplifying the apparatus and saving the cost. The apparatus was successfully used for ultra-long luminescence lifetime imaging of mouse tissue dyed with a persistent luminescence molecule. This miniaturized apparatus exhibits huge potentiality in time-resolved luminescence imaging for luminescence study and biological detection. |
Databáze: | OpenAIRE |
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