Temperature, but not excess of glycogen, regulates 'in vitro' AMPK activity in muscle samples of steer carcasses

Autor: A. Apaoblaza, Alfredo Ramírez-Reveco, Nancy Jerez-Timaure, Juan C. Slebe, Franz Villaroel-Espíndola, Alex Galaz, Pablo Strobel, Carmen Gallo
Rok vydání: 2021
Předmět:
Metabolic Processes
Glycogens
Glycogenolysis
Glycobiology
Hypothermia
AMP-Activated Protein Kinases
Biochemistry
chemistry.chemical_compound
Animal Products
Medicine and Health Sciences
Glycolysis
Post-Translational Modification
Phosphorylation
Enzyme Chemistry
Multidisciplinary
Glycogen
Chemistry
Temperature
Agriculture
Muscle Analysis
Hydrogen-Ion Concentration
Enzymes
Bioassays and Physiological Analysis
Medicine
medicine.symptom
Research Article
Signal Transduction
medicine.medical_specialty
Meat
Science
Research and Analysis Methods
Enzyme Regulation
Signs and Symptoms
Downregulation and upregulation
Internal medicine
medicine
Animals
Muscle
Skeletal
Nutrition
Biology and Life Sciences
Proteins
AMPK
In vitro
Diet
Metabolism
Endocrinology
Food
Postmortem Changes
Enzymology
Clinical Medicine
Zdroj: PLoS ONE, Vol 16, Iss 1, p e0229480 (2021)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0229480
Popis: Postmortem muscle temperature affects the rate of pH decline in a linear manner from 37.5°C to 0–2°C. The pH decline is correlated with the enzymatic degradation of glycogen to lactate and this process includes the metabolic coupling between glycogenolysis and glycolysis, and that are strongly upregulated by the AMPK. In this study, we used 12 samples previously characterized by have different muscle glycogen concentration, lactate and AMPK activity, selected from 38 steers that produced high final pH (>5.9) and normal final pH ( 0.05) and we did not detect structural differences in the polymers present in samples from both categories (p > 0.05), suggesting that postmortem AMPK activity may be highly sensitive to temperature and not toin vitrochanges in glycogen concentration (p > 0.05). Our results allow concluding that normal concentrations of muscle glycogen immediately at the time of slaughter (0.5 h) and an adequate cooling managing of carcasses are relevant to let an efficient glycogenolytic/glycolytic flow required for lactate accumulation and pH decline, through the postmortem AMPK signalling pathway.
Databáze: OpenAIRE
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