Highly sensitive signal detection of duplex dye-labelled DNA oligonucleotides in a PDMS microfluidic chip: confocal surface-enhanced Raman spectroscopic study
| Autor: | Sangyeop Lee, Won Ho Ji, Eun Kyu Lee, Yang S. Kim, Dae-Gab Gweon, Taehan Park, Sang Hoon Lee, Gi Hun Seong, Seung Yong Hwang, Jaebum Choo |
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| Rok vydání: | 2005 |
| Předmět: |
Materials science
Silver Surface Properties Confocal Biomedical Engineering Oligonucleotides Nanoparticle Bioengineering Nanotechnology Spectrum Analysis Raman Biochemistry Sensitivity and Specificity symbols.namesake chemistry.chemical_compound A-DNA Colloids Dimethylpolysiloxanes Coloring Agents Oligonucleotide Array Sequence Analysis Microscopy Confocal Oligonucleotide General Chemistry DNA Surface-enhanced Raman spectroscopy Microfluidic Analytical Techniques Fluorescence chemistry symbols Raman spectroscopy |
| Zdroj: | Lab on a chip. 5(4) |
| ISSN: | 1473-0197 |
| Popis: | Rapid and highly sensitive detection of duplex dye-labelled DNA sequences in a PDMS microfluidic channel was investigated using confocal surface enhanced Raman spectroscopy (SERS). This method does not need either an immobilization procedure or a PCR amplification procedure, which are essential for a DNA microarray chip. Furthermore, Raman peaks of each dye-labelled DNA can be easily resolved since they are much narrower than the corresponding broad fluorescence bands. To find the potential applicability of confocal SERS for sensitive bio-detection in a microfluidic channel, the mixture of two different dye-labelled (TAMRA and Cy3) sex determining Y genes, SRY and SPGY1, was adsorbed on silver colloids in the alligator teeth-shaped PDMS microfluidic channel and its SERS signals were measured under flowing conditions. Its major SERS peaks were observable down to the concentration of 10(-11) M. In the present study, we explore the feasibility of confocal SERS for the highly sensitive detection of duplex dye-labelled DNA oligonucleotides in a PDMS microfluidic chip. |
| Databáze: | OpenAIRE |
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