Immunocytochemical localisation of phospholipid hydroperoxide glutathione peroxidase in bull’s spermatogenic cells
| Autor: | Maurizio Monaci, Claudio Palombi, Giuseppe Stradaioli, Francesco Parillo, Lakamy Sylla |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
0301 basic medicine
endocrine system Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine PHGPx Immunocytochemistry Spermatozoa Bull medicine Phospholipid-hydroperoxide glutathione peroxidase Acrosome Zona pellucida reproductive and urinary physiology Fertilisation lcsh:SF1-1100 030219 obstetrics & reproductive medicine urogenital system Oocyte Sperm Spermatozoa Cell biology 030104 developmental biology medicine.anatomical_structure chemistry Biochemistry PHGPx Immunocytochemistry Animal Science and Zoology lcsh:Animal culture Spermatogenesis Immunostaining |
| Zdroj: | Italian Journal of Animal Science, Vol 13, Iss 3 (2014) |
| Popis: | Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is a selenoprotein, which protects biomembranes from oxidative damages, and it also accounts for almost the entire selenium content of mammalian testis. The present investigation was performed to localise PHGPx in the testis and in epididymal and ejaculated spermatozoa of the bull by using light and electron immunomicroscopy. The study also aimed to further clarify the possible functions of the protein in bull fertility. In the testis, spermatogenic cells of the adluminal tubular compartment showed cytoplasmatic immunostaining; whereas, in the epididymal and ejaculated spermatozoa immunostaining was specifically localised at the level of the head and mid-piece. Ultrastructural data revealed the presence of signals for PHGPx in different subcellular compartments of maturing and mature sperm (mitochondria, chromatin, nuclear envelope, acrosomes, cytoskeletal structures) suggesting that this enzyme plays versatile and important biological roles during spermatogenesis. The final localisation of the immunostaining at acrosomal level puts forward a new role of the protein which further emphasises its relevance in male reproduction: it is reported to anchor substrate of the sperm acrosome to the oocyte zona pellucida during the fertilisation process. |
| Databáze: | OpenAIRE |
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