| Autor: |
A Cotisson, Bourgeay-Causse M, G Ville, Augert C, A Favier, Arnaud J, G Meffre, J P Vuillez |
| Rok vydání: |
1994 |
| Předmět: |
|
| Zdroj: |
Scandinavian journal of clinical and laboratory investigation. 54(3) |
| ISSN: |
0036-5513 |
| Popis: |
Different methods are available for cobalamin determination in serum. Microbiological and radio ligand binding assays are the most commonly used. Kits involving non-isotopic competitive-binding assay have been recently commercialized. In the present work, cobalamins were determined in 146 patient sera, using four methods: a microbiological method, two no boil radio ligand binding assay kits (Magic B12 FOL (NB) from Ciba-Corning and SimulTRAC SNB No Boil from Becton Dickinson) and a non-isotopic kit with acridinium ester labelled cobalamin (Magic Lite from Ciba-Corning). Median (range) cobalamin concentrations in pmol l-1 were 317 (15-1291) using the microbiological method, 355 (25-3469) using the Magic Lite kit, 355 (35-2312) using the Magic B12 FOL (NB) kit and 380 (37-2021) using the SimulTRAC SNB No Boil kit. The ANOVA test indicated that differences between methods were statistically significant (p < 0.01). Competitive-binding methods gave higher results than the microbiological method. Although correlation coefficients were not excellent (0.88 < r < 0.96), the results obtained with the different methods were generally similar and confirmed that competitive methods are useful for detecting low serum concentration of vitamin B12. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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