Promotion on NLRC5 upregulating MHC-I expression by IFN-γ in MHC-I–deficient breast cancer cells
| Autor: | Ming-Zhen Zhao, Yu Sun, Xiao-Feng Jiang, Li Liu, Li-Xin Sun |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Transcriptional Activation
medicine.medical_treatment Immunology Population Genes MHC Class I Breast Neoplasms Interferon-gamma Breast cancer Immune system Cancer immunotherapy Cell Line Tumor MHC class I medicine Humans RNA Messenger education education.field_of_study biology business.industry Intracellular Signaling Peptides and Proteins Cancer Immunotherapy medicine.disease Up-Regulation Immunosurveillance MCF-7 Cells Trans-Activators biology.protein Cancer research Female business |
| Zdroj: | Immunologic Research. 67:497-504 |
| ISSN: | 1559-0755 0257-277X |
| DOI: | 10.1007/s12026-019-09111-w |
| Popis: | Breast cancer is the most dominant cancer in women and the second most frequent cancer in the general population worldwide. NLRC5 critically transactivates MHC class I (classically HLA-ABC in human) which is crucial for cancer immunosurveillance. But the expressional and functional impairments of NLRC5 have been found in many cancers as a major mechanism of immune evasion. Promotion of NLRC5 with the enhancement of MHC class I contributes to cancer immunotherapy and counteraction against cancer immune evasion. In many cancers, IFN-γ promotes the expression of MHC class I involving NLRC5; however, it is unclear in breast cancer cells. In this study, qRT-PCR, western blot, and flow cytometry were used to detect the mRNAs and proteins of NLRC5, β2m, and HLA-ABC in MHC class I–deficient human SKBR3 breast cancer cells after IFN-γ treatment. It was shown that the relative levels of NLRC5 mRNA, β2m mRNA, and HLA-ABC α heavy chain mRNA, in concentrations of 50 U/ml and 100 U/ml IFN-γ groups, were statistically increased (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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