A RP-HPLC method for quantification of diclofenac sodium released from biological macromolecules
| Autor: | Shiv Sankar Bhattacharya, Anurag Verma, Ashoke Kumar Ghosh, Amitava Ghosh, Subham Banerjee, Pronobesh Chattopadhyay |
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| Rok vydání: | 2013 |
| Předmět: |
Diclofenac
Sodium chemistry.chemical_element Galactans Biochemistry High-performance liquid chromatography Mannans chemistry.chemical_compound Drug Stability Structural Biology Plant Gums medicine Animals Molecular Biology Chromatography High Pressure Liquid Detection limit Chromatography Reverse-Phase Drug Carriers Aqueous solution Chromatography Anti-Inflammatory Agents Non-Steroidal Extraction (chemistry) General Medicine Diclofenac Sodium Reference Standards Microspheres Carboxymethyl cellulose chemistry Area Under Curve Carboxymethylcellulose Sodium Delayed-Action Preparations Calibration Locust bean gum Rabbits Blood Chemical Analysis Half-Life medicine.drug |
| Zdroj: | International Journal of Biological Macromolecules. 58:354-359 |
| ISSN: | 0141-8130 |
| Popis: | Interpenetrating network (IPN) microbeads of sodium carboxymethyl locust bean gum (SCMLBG) and sodium carboxymethyl cellulose (SCMC) containing diclofenac sodium (DS), a nonsteroidal anti-inflammatory drug, were prepared by single water-in-water (w/w) emulsion gelation process using AlCl3 as cross-linking agent in a complete aqueous environment. Pharmacokinetic study of these IPN microbeads was then carried out by a simple and feasible high-performance liquid chromatographic method with UV detection which was developed and validated for the quantification of diclofenac sodium in rabbit plasma. The chromatographic separation was carried out in a Hypersil BDS, C18 column (250 mm × 4.6 mm; 5 m). The mobile phase was a mixture of acetonitrile and methanol (70:30, v/v) at a flow rate of 1.0 ml/min. The UV detection was set at 276 nm. The extraction recovery of diclofenac sodium in plasma of three quality control (QC) samples was ranged from 81.52% to 95.29%. The calibration curve was linear in the concentration range of 20-1000 ng/ml with the correlation coefficient (r(2)) above 0.9951. The method was specific and sensitive with the limit of quantification of 20 ng/ml. In stability tests, diclofenac sodium in rabbit plasma was stable during storage and assay procedure. |
| Databáze: | OpenAIRE |
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