Capillary electrophoresis single-strand conformation polymorphism for the monitoring of gastrointestinal microbiota of chicken flocks
| Autor: | Christine Burel, S. Mallet, R. Maurice, M. Queguiner, Irène Gabriel, P. Fravalo, Michel Lessire, C. Pissavin, V. Beven |
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| Přispěvatelé: | Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES), Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), Université de Rennes (UR) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
DNA
Bacterial Male Aerobic bacteria [SDV]Life Sciences [q-bio] Biology Gut flora digestive system MICROFLORA Microbiology 03 medical and health sciences GRADIENT GEL-ELECTROPHORESIS Animals PHYLOGENETIC ANALYSIS [INFO]Computer Science [cs] Food science Animal Husbandry 030304 developmental biology 2. Zero hunger capillary electrophoresis single-strand conformation polymorphism 0303 health sciences Polymorphism Genetic Bacteria SALERS CHEESE gut microbiota 030306 microbiology Electrophoresis Capillary REGISTERED DESIGNATION CECAL MICROBIOTA Single-strand conformation polymorphism General Medicine biology.organism_classification 16S ribosomal RNA INTESTINAL BACTERIAL COMMUNITY Hatchery SSCP BROILER-CHICKENS Gastrointestinal Tract GENETIC PROFILES Animal Science and Zoology Cloaca Flock Chickens |
| Zdroj: | Poultry Science Poultry Science, Poultry Science Association, 2012, 91 (9), pp.2294-2304. ⟨10.3382/ps.2011-01911⟩ Poultry Science, 2012, 91 (9), pp.2294-2304. ⟨10.3382/ps.2011-01911⟩ |
| ISSN: | 0032-5791 |
| DOI: | 10.3382/ps.2011-01911⟩ |
| Popis: | International audience; The objective of the present study was to evaluate the capillary electrophoresis single-strand conformation polymorphism (CE-SSCP) to characterize poultry gut microbiota and the ability of this molecular method to detect modifications related to rearing conditions to be used as an epidemiological tool. The V3 region of the 16S rRNA gene was selected as the PCR target. Our results showed that this method provides reproducible data. The microbiota analysis of individuals showed that variability between individual fingerprints was higher for ileum and cloaca than for ceca. However, pooling the samples decreased this variability. To estimate the variability within and between farms, we compared molecular gut patterns of animals from the same hatchery reared under similar conditions and fed the same diet in 2 separate farms. Total aerobic bacteria, coliforms, and lactic acid bacteria were enumerated using conventional bacteriological methods. A significant difference was observed for coliforms present in the ceca and the cloaca depending on the farm. Ileal contents fingerprints were more closely related to those of cloacal contents than to those of ceca contents. When comparing samples from the 2 farms, a specific microbiota was highlighted for each farm. For each gut compartment, the microbiota fingerprints were joined in clusters according to the farm. Thus, this rapid and potentially high-throughput method to obtain gut flora fingerprints is sensitive enough to detect a "farm effect" on the balance of poultry gut microbiota despite the birds being fed the same regimens and reared under similar conditions. |
| Databáze: | OpenAIRE |
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