Culture of human tumor infiltrating lymphocytes in hollow fiber bioreactors
| Autor: | Steven A. Rosenberg, Paul Aebersold, Richard A. Knazek, Yan Wan Wu |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Cytotoxicity
Immunologic Interleukin 2 Tumor-infiltrating lymphocytes T-Lymphocytes Lymphocyte Immunology hemic and immune systems chemical and pharmacologic phenomena Biology Molecular biology Autologous tumor cell Culture Media Tissue culture medicine.anatomical_structure Cell culture medicine Bioreactor Humans Immunology and Allergy Fiber Melanoma Cells Cultured medicine.drug |
| Zdroj: | Journal of Immunological Methods. 127:29-37 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(90)90337-u |
| Popis: | Human tumor infiltrating lymphocytes (TIL) from metastatic melanoma of six patients were grown using a new hollow fiber bioreactor system. After inoculating 0.35–10 × 10 8 TIL into the extra-fiber space (EFS), each Cellmax bioreactor was perfused with AIM-V medium, supplemented with rIL-2. The cells subsequently expanded 124–1170-fold to yield 1.5–5.4 × 10 10 TIL over a 14–32 day period. TIL were flushed from the EFS using 200 ml medium and possessed an average viability = 91%. The phenotype and the autologous tumor cell lytic capacity of these TIL were similar to those of TIL grown in the currently used gas-permeable culture bags. Tissue culture media use averaged 4.3 liters/10 10 TIL harvested. The TIL of one patient were re-expanded twice from cells remaining within the same bioreactor after harvest suggesting that one bioreactor cartridge could be used for repetitive, periodic studies. An estimated 80% decrease in technical time expended and in incubator space requirements were realized using this methodology. Cell culture on hollow fibers appears to be a useful method for producing large quantities of primary human lymphocytes for experimental, and perhaps, therapeutic needs. |
| Databáze: | OpenAIRE |
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