GALA peptide improves the potency of nanobody–drug conjugates by lipid-induced helix formation
| Autor: | Zhengshuang Xu, Xiao Chun Guo, Yong Juan Zhao, Hon Cheung Lee, Ya Jie Chen, Jian Yuan Yang, Qi Wen Deng, Li Wang, Ting Li |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Antineoplastic Agents
Peptide Nanoconjugates 010402 general chemistry Endocytosis 01 natural sciences Catalysis law.invention law Cell Line Tumor Amphiphile Materials Chemistry Humans Cytotoxicity chemistry.chemical_classification 010405 organic chemistry Chemistry Metals and Alloys General Chemistry Recombinant Proteins 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Helix Ceramics and Composites Biophysics Recombinant DNA Peptides Oligopeptides Alpha helix Single-Chain Antibodies Conjugate |
| Zdroj: | Chemical Communications. 57:1434-1437 |
| ISSN: | 1364-548X 1359-7345 |
| DOI: | 10.1039/d0cc07706b |
| Popis: | A novel nanobody-drug conjugate (NDC) was constructed by incorporating an amphipathic peptide, GALA, which improved the cytotoxicity by one to two orders of magnitude. Mechanistic studies demonstrate that tethering to lipids induces GALA to form a helix, which dramatically enhances endocytosis. Our work provides a general strategy not only for improving the anti-cancer efficacy of protein-drug conjugates but also for increasing the efficiency of other types of endocytosis-dependent cell delivery. |
| Databáze: | OpenAIRE |
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