Mitochondrial nucleoid morphology and respiratory function are altered in Drp1-deficient HeLa cells
| Autor: | Azusa Ota, Naotada Ishihara, Takaya Ishihara |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Dynamins
endocrine system Mitochondrial DNA Cell Respiration Mitochondrion Biochemistry DNA Mitochondrial Mitochondrial Dynamics HeLa 03 medical and health sciences Gene Knockout Techniques 0302 clinical medicine Nucleoid Humans Respiratory function RNA Small Interfering Molecular Biology 030304 developmental biology Mitochondrial nucleoid 0303 health sciences biology Chemistry General Medicine biology.organism_classification Cell biology Mitochondria mitochondrial fusion Microscopy Fluorescence Mitochondrial fission CRISPR-Cas Systems 030217 neurology & neurosurgery HeLa Cells |
| Zdroj: | Journal of biochemistry. 167(3) |
| ISSN: | 1756-2651 |
| Popis: | Mitochondria are dynamic organelles that frequently divide and fuse with each other. The dynamin-related GTPase protein Drp1 has a key role in mitochondrial fission. To analyse the physiological roles of Drp1 in cultured human cells, we analysed Drp1-deficient HeLa cells established by genome editing using CRISPR/Cas9. Under fluorescent microscopy, not only mitochondria were elongated but their DNA (mtDNA) nucleoids were extremely enlarged in bulb-like mitochondrial structures (‘mito-bulbs’) in the Drp1-deficient HeLa cells. We further found that respiratory activity, as measured by oxygen consumption rates, was severely repressed in Drp1-deficient HeLa cells and that this was reversible by the co-repression of mitochondrial fusion factors. Although mtDNA copy number was not affected, several respiratory subunits were repressed in Drp1-deficient HeLa cells. These results suggest that mitochondrial fission is required for the maintenance of active respiratory activity and the morphology of mtDNA nucleoids in human cells. |
| Databáze: | OpenAIRE |
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