Quadruplexes of human telomere DNA analogs designed to contain G:A:G:A, G:G:A:A, and A:A:A:A tetrads
| Autor: | Janos Sagi, Michaela Vorlíčková, Martin Tomaško, Daniel Renčiuk |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Circular dichroism
Stereochemistry Molecular Sequence Data Biophysics Antiparallel (biochemistry) G-quadruplex Nucleic Acid Denaturation Biochemistry Biomaterials chemistry.chemical_compound Humans heterocyclic compounds Dimethyl Sulfoxide Tetrad Base Sequence Chemistry Circular Dichroism fungi Organic Chemistry Sodium Temperature General Medicine DNA Telomere G-Quadruplexes Solutions Intramolecular force Potassium Electrophoresis Polyacrylamide Gel |
| Zdroj: | Biopolymers. 93(10) |
| ISSN: | 0006-3525 |
| Popis: | Replacement of two to four guanines by adenines in the human telomere DNA repeat dG3(TTAG3)3 did not hinder the formation of quadruplexes if the substitutions took place in the terminal tetrad bridged by the diagonal loop of the intramolecular antiparallel three-tetrad scaffold, as proved by CD and PAGE in both Na+ and K+ solutions. Thermodynamic data showed that, in Na+ solution, the dG3(TTAG3)3 quadruplex was destabilized, the least by the two G:A:G:A tetrads, the most by the G:G:A:A tetrad in which the adenosines replaced syn-guanosines. In physiological K+ solution, the highest destabilization was caused by the 4A tetrad. In K+, only the unmodified dG3(TTAG3)3 quadruplex rearranged into a K+-dependent quadruplex form, none of the multiple adenine-modified structures did so. This may imply biological consequences for nonrepaired A-for-G mutations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text