Investigation of diazo‐derivatization of bisphenol A and its applicability for quantitation in food safety inspections using high‐performance liquid chromatography
| Autor: | Libo Wang, Hong Yang, Zhongrui Liu, Liwen Sun, Xin Zheng, Yangyang Li |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
endocrine system
Bisphenol A Food Safety Clinical Biochemistry Food Contamination 030226 pharmacology & pharmacy 01 natural sciences Biochemistry High-performance liquid chromatography Analytical Chemistry Beverages 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Phenols Tap water Limit of Detection Drug Discovery Benzhydryl Compounds Derivatization Molecular Biology Chromatography High Pressure Liquid Pharmacology Detection limit Chromatography urogenital system Chemistry 010401 analytical chemistry Food Packaging Reproducibility of Results General Medicine 0104 chemical sciences Food packaging Distilled water Reagent Linear Models hormones hormone substitutes and hormone antagonists |
| Zdroj: | Biomedical Chromatography. 33:e4419 |
| ISSN: | 1099-0801 0269-3879 |
| Popis: | Bisphenol A (BPA) is a common additive in food packaging materials, and many methods have been discovered to limit BPA for the sake of food safety. This study aims to find a more sensitive quantification method for BPA. It was determined that a diazo derivative of BPA could be obtained when reacted with a stable and inexpensive reagent, p-methylaniline, exhibiting an increased response in the ultraviolet spectrum than that of nonreacted BPA. Based on this discovery, an accurate method of BPA quantitation was established using a precolumn derivatization HPLC method. The results of this study showed the low limit of detection and the limit of quantitation for BPA were 3.6 and 10.9 pg/mL on-column, respectively. This method is proven to have much higher sensitivity and a lower limit of detection than direct HPLC determination methods. This method is robust, easily handled, sensitive and cost-effective for the quantitation of BPA. In addition, the broad application of this method to determine BPA content in plastic bottled drinking water, distilled water, tap water, milk, Sprite and grape juice was demonstrated in this paper. |
| Databáze: | OpenAIRE |
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