Elaidic acid sustains LPS and TNF-α induced ICAM-1 and VCAM-I expression on human bone marrow endothelial cells (HBMEC)
| Autor: | Fariba Ghanbari, Ali Mostafaie, Nima Sanadgol, Kamran Mansouri, Ali Bidmeshkipour, Gholamreza Bahrami |
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| Rok vydání: | 2010 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide Blotting Western Clinical Biochemistry Cell Vascular Cell Adhesion Molecule-1 Bone Marrow Cells Enzyme-Linked Immunosorbent Assay Oleic Acids chemistry.chemical_compound medicine Humans VCAM-1 Cells Cultured ICAM-1 Tumor Necrosis Factor-alpha Cell adhesion molecule Endothelial Cells General Medicine Intercellular Adhesion Molecule-1 Elaidic acid Molecular biology Endothelial stem cell Oleic acid medicine.anatomical_structure chemistry Biochemistry Oleic Acid |
| Zdroj: | Clinical Biochemistry. 43:968-972 |
| ISSN: | 0009-9120 |
| Popis: | Objectives Elaidic acid, the predominant trans-fatty acid in industrially hydrogenated oils, exists on high levels in Iranian hydrogenated oils and margarines. This study was undertaken to investigate the effect of elaidic acid and its cis-counterpart oleic acid on expression of ICAM-1 and VCAM-1 on human bone marrow endothelial cells (HBMECs). Design and methods HBMEC were pre-treated with TNF-α or LPS for induction of the adhesion molecules expression, and then treated with elaidic acid or oleic acid. Soluble and cell associated forms of ICAM-1 and VCAM-1 were quantified by ELISA and Western blot. Results Our findings indicated that oleic acid suppresses VCAM-1 and ICAM-1 expression on HBMEC near to the basal level. Conversely, elaidic acid maintained the level of VCAM-1 and ICAM-1 up-regulated by TNF-α or LPS. Conclusions It is suggested that elaidic acid could keep the HBMEC at the stimulated phenotype. These findings provide further support on the detrimental effects of elaidic acid in promotion and induction of cardiovascular diseases (CVD). |
| Databáze: | OpenAIRE |
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