Rapid MALDI-MS Assays for Drug Quantification in Biological Matrices: Lessons Learned, New Developments, and Future Perspectives
| Autor: | Jürgen Burhenne, Inka Zörnig, Stefan M. Pfister, Rémi Longuespée, Kristian W. Pajtler, Esra Yildirim, Walter E. Haefeli, Julia Benzel, Dirk Jäger, Margaux Fresnais, Seda Karabulut |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Drug
Bioanalysis Analyte Maldi ms Computer science media_common.quotation_subject therapeutic drug monitoring Pharmaceutical Science Mass spectrometry 01 natural sciences Article Analytical Chemistry lcsh:QD241-441 03 medical and health sciences Matrix (mathematics) lcsh:Organic chemistry Drug Discovery medicine Humans Instrumentation (computer programming) Physical and Theoretical Chemistry MALDI 030304 developmental biology media_common mass spectrometry targeted quantification 0303 health sciences medicine.diagnostic_test 010401 analytical chemistry Organic Chemistry 0104 chemical sciences Pharmaceutical Preparations Chemistry (miscellaneous) Therapeutic drug monitoring Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Molecular Medicine Biochemical engineering Drug Monitoring Crystallization |
| Zdroj: | Molecules Molecules, Vol 26, Iss 1281, p 1281 (2021) Volume 26 Issue 5 |
| ISSN: | 1420-3049 |
| Popis: | Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has rarely been used in the field of therapeutic drug monitoring, partly because of the complexity of the ionization processes between the compounds to be quantified and the many MALDI matrices available. The development of a viable MALDI-MS method that meets regulatory guidelines for bioanalytical method validation requires prior knowledge of the suitability of (i) the MALDI matrix with the analyte class and properties for ionization, (ii) the crystallization properties of the MALDI matrix with automation features, and (iii) the MS instrumentation used to achieve sensitive and specific measurements in order to determine low pharmacological drug concentrations in biological matrices. In the present hybrid article/white paper, we review the developments required for the establishment of MALDI-MS assays for the quantification of drugs in tissues and plasma, illustrated with concrete results for the different steps. We summarize the necessary parameters that need to be controlled for the successful development of fully validated MALDI-MS methods according to regulatory authorities, as well as currently unsolved problems and promising ways to address them. Finally, we propose an expert opinion on future perspectives and needs in order to establish MALDI-MS as a universal method for therapeutic drug monitoring. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|