Characterization of muscarinic acetylcholine receptors expressed by an atrial cell line derived from a transgenic mouse tumor
| Autor: | C. L. Gartside, Neil M. Nathanson, Stephen D. Hauschka, M E Morton, Craig L. Brumwell |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Physiology Antigens Polyomavirus Transforming Mice Transgenic Simian virus 40 Biology Heart Neoplasms Mice GTP-Binding Proteins Internal medicine Muscarinic acetylcholine receptor M5 Muscarinic acetylcholine receptor medicine Muscarinic acetylcholine receptor M4 Animals Receptor Cells Cultured Guanylyl Imidodiphosphate Myocardium Muscarinic antagonist Muscarinic acetylcholine receptor M3 Muscarinic acetylcholine receptor M2 Muscarinic acetylcholine receptor M1 Receptors Muscarinic Cell biology Endocrinology Cardiology and Cardiovascular Medicine medicine.drug |
| Zdroj: | Circulation research. 74(4) |
| ISSN: | 0009-7330 |
| Popis: | The properties of muscarinic acetylcholine receptors in the cell line MCM1, derived from an SV40 T-antigen-induced atrial tumor in a transgenic mouse, were determined. Binding studies using the nonselective muscarinic antagonist [3H]quinuclidinyl benzilate, the M1-selective antagonist pirenzepine, and the M2-selective antagonist AFDX-116 indicate that the receptors have the pharmacological properties of the cardiac (M2) receptor subtype. The receptors could be immunoprecipitated with a monoclonal antibody specific for the cardiac receptor, thus confirming the identity of the receptors expressed in these cells. The types of G proteins expressed in the cells were determined by Northern blot analyses: mRNA encoding the alpha subunits of Gs, G(o), and Gi-2, but not Gi-1 or Gi-3, were detected, consistent with previous observations of neonatal mammalian atria. The muscarinic receptors were functionally active, as demonstrated by the ability of the agonist to stimulate phosphoinositide turnover and to inhibit adenylyl cyclase activity. The availability of a mammalian atrial cell line that continues to express the appropriate functionally coupled subtype of muscarinic receptor may provide a useful system for the investigation of the regulation of expression and function of cardiac muscarinic receptors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|