Labeling mesenchymal cells with DMSA-coated gold and iron oxide nanoparticles: assessment of biocompatibility and potential applications

Autor: Guilherme A. Ferreira, D. M. Oliveira, Ricardo Bentes Azevedo, Emília Celma de Oliveira Lima, Renata C. Silva, Jaqueline Rodrigues da Silva, Luisa H. A. Silva
Rok vydání: 2016
Předmět:
0301 basic medicine
Cellular differentiation
Pharmaceutical Science
Medicine (miscellaneous)
02 engineering and technology
Applied Microbiology and Biotechnology
Ferric Compounds
chemistry.chemical_compound
Mice
Magnetic targeting
Cytotoxic T cell
Cytotoxicity
Magnetite Nanoparticles
Nanopartículas
Cell Differentiation
021001 nanoscience & nanotechnology
Mitochondria
Computed microtomography
Colloidal gold
Cell Tracking
Iron oxide nanoparticle
Molecular Medicine
Biocompatibility
0210 nano-technology
Células-tronco
Succimer
Iron oxide nanoparticles
Adult
Adolescent
Cell Survival
Primary Cell Culture
Silicosis
Biomedical Engineering
Bioengineering
Nanotechnology
03 medical and health sciences
Animals
Humans
Gold nanoparticles
Particle Size
Dental Pulp
Cell Proliferation
Staining and Labeling
Research
Mesenchymal stem cell
Biocompatibilidade
Mesenchymal Stem Cells
X-Ray Microtomography
Mice
Inbred C57BL
030104 developmental biology
chemistry
Cell culture
DMSA-nanoparticles
Biophysics
Gold
Zdroj: Journal of Nanobiotechnology
Repositório Institucional da UnB
Universidade de Brasília (UnB)
instacron:UNB
ISSN: 1477-3155
Popis: Background Nanoparticles’ unique features have been highly explored in cellular therapies. However, nanoparticles can be cytotoxic. The cytotoxicity can be overcome by coating the nanoparticles with an appropriated surface modification. Nanoparticle coating influences biocompatibility between nanoparticles and cells and may affect some cell properties. Here, we evaluated the biocompatibility of gold and maghemite nanoparticles functionalized with 2,3-dimercaptosuccinic acid (DMSA), Au-DMSA and γ-Fe2O3-DMSA respectively, with human mesenchymal stem cells. Also, we tested these nanoparticles as tracers for mesenchymal stem cells in vivo tracking by computed tomography and as agents for mesenchymal stem cells magnetic targeting. Results Significant cell death was not observed in MTT, Trypan Blue and light microscopy analyses. However, ultra-structural alterations as swollen and degenerated mitochondria, high amounts of myelin figures and structures similar to apoptotic bodies were detected in some mesenchymal stem cells. Au-DMSA and γ-Fe2O3-DMSA labeling did not affect mesenchymal stem cells adipogenesis and osteogenesis differentiation, proliferation rates or lymphocyte suppression capability. The uptake measurements indicated that both inorganic nanoparticles were well uptaken by mesenchymal stem cells. However, Au-DMSA could not be detected in microtomograph after being incorporated by mesenchymal stem cells. γ-Fe2O3-DMSA labeled cells were magnetically responsive in vitro and after infused in vivo in an experimental model of lung silicosis. Conclusion In terms of biocompatibility, the use of γ-Fe2O3-DMSA and Au-DMSA as tracers for mesenchymal stem cells was assured. However, Au-DMSA shown to be not suitable for visualization and tracking of these cells in vivo by standard computed microtomography. Otherwise, γ-Fe2O3-DMSA shows to be a promising agent for mesenchymal stem cells magnetic targeting.
Databáze: OpenAIRE
Externí odkaz: