Cytoneme-mediated cell-cell contacts for hedgehog reception
| Autor: | Isabel Guerrero, Laura González-Méndez, Irene Seijo-Barandiarán |
|---|---|
| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Fundación Ramón Areces |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell signaling animal structures QH301-705.5 Science Morphogenesis Receptors Cell Surface Hedgehog signaling Cell Communication Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Animals Drosophila Proteins Hedgehog Proteins cytonemes Pseudopodia Biology (General) Hedgehog D. melanogaster General Immunology and Microbiology General Neuroscience General Medicine Anatomy Hedgehog signaling pathway Cell biology Developmental Biology and Stem Cells 030104 developmental biology morphogen gradient embryonic structures Medicine Drosophila Stem cell Research Article Morphogen Cytoneme |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname eLife, Vol 6 (2017) eLife |
| Popis: | Morphogens regulate tissue patterning through their distribution in concentration gradients. Emerging research establishes a role for specialized signalling filopodia, or cytonemes, in morphogen dispersion and signalling. Previously we demonstrated that Hedgehog (Hh) morphogen is transported via vesicles along cytonemes emanating from signal-producing cells to form a gradient in Drosophila epithelia. However, the mechanisms for signal reception and transfer are still undefined. Here, we demonstrate that cytonemes protrusding from Hh-receiving cells contribute to Hh gradient formation. The canonical Hh receptor Patched is localized in these cellular protrusions and Hh reception takes place in membrane contact sites between Hh-sending and Hh-receiving cytonemes. These two sets of cytonemes have similar dynamics and both fall in two different dynamic behaviours. Furthermore, both the Hh co-receptor Interference hedgehog (Ihog) and the glypicans are critical for this cell-cell cytoneme mediated interaction. These findings suggest that the described contact sites might facilitate morphogen presentation and reception. Spanish Ministry of Economy and Competitiveness (MINECO), and by a personal and by institutional grants from the Fundación Ramón Areces |
| Databáze: | OpenAIRE |
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