The Cell Cycle Regulatory Factor TAF1 Stimulates Ribosomal DNA Transcription by Binding to the Activator UBF
| Autor: | Pierluigi Scalia, Tiffany Bui, Lucio Comai, Chih-Yin Lin, JoAnn Tuan |
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| Rok vydání: | 2002 |
| Předmět: |
Transcription
Genetic Chromosomal Proteins Non-Histone Nucleolus Ribosome biogenesis Biology Transfection DNA Ribosomal Ribosome General Biochemistry Genetics and Molecular Biology RNA Polymerase I Transcription (biology) Two-Hybrid System Techniques RNA polymerase I Animals Humans Histone Chaperones Cells Cultured Histone Acetyltransferases Regulator gene TATA-Binding Protein Associated Factors Cell-Free System Agricultural and Biological Sciences(all) General transcription factor Biochemistry Genetics and Molecular Biology(all) Cell Cycle Molecular biology Recombinant Proteins DNA-Binding Proteins TAF1 Mutation Transcription Factor TFIID RNA Polymerase II General Agricultural and Biological Sciences Pol1 Transcription Initiation Complex Proteins HeLa Cells Transcription Factors |
| Zdroj: | Current Biology. 12:2142-2146 |
| ISSN: | 0960-9822 |
| DOI: | 10.1016/s0960-9822(02)01389-1 |
| Popis: | Control of ribosome biogenesis is a potential mechanism for the regulation of cell size during growth [1, 2], and a key step in regulating ribosome production is ribosomal RNA synthesis by RNA polymerase I (Pol I) [3, 4]. In humans, Pol I transcription requires the upstream binding factor UBF and the selectivity factor SL1 to assemble coordinately on the promoter [5–7]. UBF is an HMG box-containing factor that binds to the rDNA promoter and activates Pol I transcription through its acidic carboxy-terminal tail [8, 9]. Using UBF (284–670) as bait in a yeast two-hybrid screen, we have identified an interaction between UBF and TAF1, a factor involved in the transcription of cell cycle and growth regulatory genes [10]. Coimmunoprecipitation and protein-protein interaction assays confirmed that TAF1 binds to UBF. Confocal microscopy showed that TAF1 colocalizes with UBF in Hela cells, and cell fractionation experiments provided further evidence that a portion of TAF1 is localized in the nucleolus, the organelle devoted to ribosomal DNA transcription. Cotransfection and in vitro transcription assays showed that TAF1 stimulates Pol I transcription in a dosage-dependent manner. Thus, TAF1 may be involved in the coordinate expression of Pol I- and Pol II-transcribed genes required for protein biosynthesis and cell cycle progression. |
| Databáze: | OpenAIRE |
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