Profiling of influenza viruses by high-throughput carbohydrate membrane array
| Autor: | Ih-Jen Su, Weng I. Lao, Jen Ren Wang, Chuan Fa Chang, Yu Dai Kuo, Tsung Chain Chang, Ya Fang Wang, Chun-Hung Lin |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Glycan
Hemagglutinin Glycoproteins Influenza Virus Plasma protein binding medicine.disease_cause Antibodies law.invention Antigen law Polysaccharides Lectins Drug Discovery medicine Pharmacology Membranes biology Microarray analysis techniques Proteins Carbohydrate Microarray Analysis Influenza A virus subtype H5N1 Influenza B virus Biochemistry biology.protein Recombinant DNA Molecular Medicine Antibody Protein Binding |
| Zdroj: | Future medicinal chemistry. 3(3) |
| ISSN: | 1756-8927 |
| Popis: | Background: Carbohydrate–protein interactions participate in many biological functions. To characterize the binding interactions represents a longstanding challenge. Method: We developed a glycan membrane array to study the interactions of carbohydrates with lectins, proteins and viruses, including 17 lectins, four antibodies (that are specific to different Lewis antigens), one recombinant H5N1 hemagglutinin, and five influenza B clinical isolates. Results: The results were found to be comparable to previous reports, indicating the efficacy and reliability of our developed method. Conclusion: This carbohydrate membrane array represents a convenient, reliable and low-cost method to examine the carbohydrate-binding features of various proteins, high-throughput drug screening and the glycan-binding surveillance of influenza viruses. |
| Databáze: | OpenAIRE |
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