Targeted expression of RALT in mouse skin inhibits epidermal growth factor receptor signalling and generates a Waved-like phenotype
| Autor: | Laura Tatangelo, Oreste Segatto, Costanza Ballarò, Anna Maria Salvatore, Cecilia Tiveron, Sara Ceccarelli, Stefano Alemà |
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| Rok vydání: | 2005 |
| Předmět: |
Keratinocytes
Time Factors ErbB Ligands Biochemistry ErbB Receptors Mice feedback inhibition RNA interference Epidermal growth factor Epidermal growth factor receptor Transgenes Phosphorylation Promoter Regions Genetic Cells Cultured Skin Gene knockdown biology integumentary system Intracellular Signaling Peptides and Proteins Oncogene Proteins v-erbB Cell biology Phenotype Signal transduction Signal Transduction Heterozygote Transgene Scientific Report Blotting Western Immunoblotting Mice Transgenic keratinocyte Cell Line Open Reading Frames Genetics Animals Humans Molecular Biology Alleles Cell Proliferation Dose-Response Relationship Drug Epidermal Growth Factor Molecular biology Bromodeoxyuridine Vibrissae biology.protein NIH 3T3 Cells RALT Hair |
| Zdroj: | EMBO reports 6 (2005): 755–761. doi:10.1038/sj.embor.7400458 info:cnr-pdr/source/autori:Ballaro C, Ceccarelli S, Tiveron C, Tatangelo L, Salvatore AM, Segatto O, Alema S./titolo:Targeted expression of RALT in mouse skin inhibits epidermal growth factor receptor signalling and generates a Waved-like phenotype/doi:10.1038%2Fsj.embor.7400458/rivista:EMBO reports (Print)/anno:2005/pagina_da:755/pagina_a:761/intervallo_pagine:755–761/volume:6 |
| DOI: | 10.1038/sj.embor.7400458 |
| Popis: | Although it has been clearly established that negative feedback loops have a fundamental role in the regulation of epidermal growth factor receptor (EGFR) signalling in flies, their role in the regulation of mammalian EGFR has been inferred only recently from in vitro studies. Here, we report on the forced expression of RALT/MIG-6, a negative feedback regulator of ErbB receptors, in mouse skin. A RALT transgene driven by the K14 promoter generated a dose-dependent phenotype resembling that caused by hypomorphic and antimorphic Egfr alleles—that is, wavy coat, curly whiskers and open eyes at birth. Ex vivo keratinocytes from K14-RALT mice showed reduced biochemical and biological responses when stimulated by ErbB ligands. Conversely, knockdown of RALT by RNA interference enhanced ErbB mitogenic signalling. Thus, RALT behaves as a suppressor of EGFR signalling in mouse skin. |
| Databáze: | OpenAIRE |
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