Altered mitochondrial quality control signaling in muscle of old gastric cancer patients with cachexia
| Autor: | Anna Picca, Giovanni Battista Doglietto, Emanuele Marzetti, Maria Lorenzi, Fausto Rosa, Francesco Landi, Maurizio Bossola, Matteo Cesari, Fabio Pacelli, Fabiana Tanganelli, Roberto Bernabei, Riccardo Calvani, Marco Galli |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Aging Cachexia Settore MED/18 - CHIRURGIA GENERALE Peroxisome Proliferator-Activated Receptors Biochemistry Muscle wasting Parkin Endocrinology Mitophagy Kinase Mitochondrial Turnover Skeletal Middle Aged Mitochondria Italy Muscle Female Signal Transduction Adult medicine.medical_specialty Bioenergetics Adenocarcinoma Biology 03 medical and health sciences Mitofusin-2 Mitochondrial biogenesis Stomach Neoplasms Internal medicine Genetics medicine Humans Muscle Skeletal Molecular Biology Aged Mitochondrial dynamics Cell Biology Settore MED/09 - MEDICINA INTERNA Cancer TFAM medicine.disease Mitochondria Muscle Oxidative Stress Cross-Sectional Studies 030104 developmental biology Case-Control Studies Energy Metabolism |
| Zdroj: | Experimental Gerontology. 87:92-99 |
| ISSN: | 0531-5565 |
| DOI: | 10.1016/j.exger.2016.10.003 |
| Popis: | Mitochondrial dysfunction is involved in the loss of muscle featuring both aging and cancer cachexia (CC). Whether mitochondrial quality control (MQC) is altered in skeletal myocytes of old patients with CC is unclear. The present investigation therefore sought to preliminarily characterize MQC pathways in muscle of old gastric cancer patients with cachexia. The study followed a case-control cross-sectional design. Intraoperative biopsies of the rectus abdominis muscle were obtained from 18 patients with gastric adenocarcinoma (nine with CC and nine non-cachectic) and nine controls, and assayed for the expression of a set of MQC mediators. The mitofusin 2 expression was reduced in cancer patients compared with controls, independent of CC. Fission protein 1 was instead up-regulated in CC patients relative to the other groups. The mitophagy regulators PTEN-induced putative kinase 1 and Parkin were both down-regulated in cancer patients compared with controls. The ratio between the protein content of the lipidated and non-lipidated forms of microtubule-associated protein 1 light chain 3B was lower in CC patients relative to controls and non-cachectic cancer patients. Finally, the expression of autophagy-associated protein 7, lysosome-associated membrane protein 2, peroxisome proliferator-activated receptor-γ coactivator-1α, and mitochondrial transcription factor A was unvarying among groups. Collectively, our findings indicate that, in old patients with gastric cancer, cachexia is associated with derangements of the muscular MQC axis at several checkpoints: mitochondrial dynamics, mitochondrial tagging for disposal, and mitophagy signaling. Further investigations are needed to corroborate these preliminary findings and determine whether MQC pathways may become target for future interventions. |
| Databáze: | OpenAIRE |
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