Retracted: The Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas
| Autor: | Longevity Omac |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Vascular Endothelial Growth Factor A
Aging Article Subject Ultraviolet Rays Interleukin-1beta Nitric Oxide Synthase Type II Oxidative phosphorylation Biochemistry Cornea chemistry.chemical_compound Re-Epithelialization Animals Wound Healing QH573-671 Trehalose Rabbit (nuclear engineering) Cell Biology General Medicine Molecular biology Retraction Oxidative Stress chemistry Keratins Tyrosine Female Rabbits Cytology Reactive Oxygen Species Corneal Injuries DNA Damage |
| Zdroj: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2020 (2020) |
| ISSN: | 1942-0994 1942-0900 |
| DOI: | 10.1155/2020/4128985 |
| Popis: | Our previous research revealed that trehalose, a nonreducing disaccharide of glucose and an important stress responsive factor, proved to have anti-inflammatory, antiapoptotic, and particularly antioxidant properties in UVB-irradiated corneas. Trehalose reduced oxidative stress in corneas induced by UVB irradiation, by means of a decrease in the antioxidant/prooxidant imbalance in the corneal epithelium. In this study, we demonstrate that trehalose of 3% or 6% concentration in eye drops directly decreases oxidative stress in UVB-irradiated corneas, by removing the excessive amount of reactive oxygen species (ROS). Trehalose drops applied on corneas during UVB irradiation once daily for four days resulted in a reduction or even absence of the oxidative stress, DNA damage, and peroxynitrite formation (detected by nitrotyrosine residues), seen in buffer-treated corneas. Furthermore, trehalose treatment applied curatively after repeated irradiation for the subsequent fourteen days led to the renewal of corneal transparency and significant suppression or even absence of neovascularization. This was in contrast to buffer-treated irradiated corneas, where the intracorneal inflammation was developed and the untransparent corneas were vascularized. In conclusion, the treatment of UVB-irradiated corneas with trehalose eye drops removed the excessive amount of ROS in the corneal epithelium, leading to the suppression of oxidative stress and favorable corneal healing. The 6% trehalose showed a higher intensive antioxidant effect. |
| Databáze: | OpenAIRE |
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