Leucocyte recruitment induced by type II phospholipases A2 into the rat pleural cavity
| Autor: | R C de Castro, Marcos H. Toyama, Edson Antunes, Sergio Marangoni, JoséR. Giglio, G. De Nucci, Elen C.T. Landucci |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
Serotonin Neutrophils Reptilian Proteins Toxicology Group II Phospholipases A2 Peripheral blood mononuclear cell Cell Degranulation Phospholipases A Leukocyte Count chemistry.chemical_compound Phospholipase A2 Crotalid Venoms medicine Animals p-Methoxy-N-methylphenethylamine Mast Cells Rats Wistar biology Degranulation Acetophenones Pleural cavity medicine.disease Molecular biology In vitro Rats Eosinophils Chemotaxis Leukocyte medicine.anatomical_structure chemistry Pleurisy Toxicity Immunology biology.protein Pleura Histamine |
| Zdroj: | Toxicon. 38:1773-1785 |
| ISSN: | 0041-0101 |
| DOI: | 10.1016/s0041-0101(00)00107-0 |
| Popis: | Bothropstoxin-I (BthTX-I) and bothropstoxin-II (BthTX-II) are Lys-49 and Asp-49 phospholipases A(2) (PLA(2)s), respectively, isolated from Bothrops jararacussu venom. Piratoxin-I (PrTX-I) is a Lys-49 PLA(2) isolated from Bothrops pirajai venom. In this study, the ability of BthTX-I, BthTX-II and PrTX-I to recruit leucocytes into the rat pleural cavity and potential mechanisms underlying this effect were investigated. Intrapleural injection of either BthTX-I or PrTX-I (10-100 microg/cavity each) caused a significant leucocyte infiltration at 12 h after injection. The maximal cell migration was observed with the dose of 30 microg/cavity (14.9+/-15.5 and 17.6+/-1. 6x10(6) cells/cavity, respectively). Leucocyte counts consisted mainly of mononuclear cells, but significant amounts of neutrophils and eosinophils were also observed. Intrapleural injection of BthTX-II (10-100 microg/cavity) caused a marked leucocyte infiltration at 6 and 12 h after injection. The maximal response was observed with the dose of 100 microg/cavity (57.3+/-3.4x10(6) cells/cavity, 6 h). The leucocyte counts were mainly composed of neutrophils and mononuclear cells. The treatment of either BthTX-I (30 microg/cavity, 12 h) or BthTX-II (30 microg/cavity, 6 h) with the PLA(2) inhibitor p-bromophenacyl bromide (p-BPB) had no effect on the total and differential leucocyte counts induced by these proteins. The same treatment partially reduced the PrTX-I-induced pleural leucocyte infiltration. In rats depleted of the histamine and 5-hydroxytryptamine (5-HT) stores by chronic treatment with compound 48/80, the total leucocyte counts in response to BthTX-I, BthTX-II and PrTX-I was not significantly affected compared to control animals. In addition, BthTX-I, BthTX-II and PrTX-I (100 microg/ml each) significantly degranulated pleural mast cells in vitro leading to the release of [(14)C]5-hydroxytryptamine ([(14)C]5-HT). p-BPB and heparin (50 IU/ml) significantly reduced the [(14)C]5-HT release induced by these PLA(2)s. Our results demonstrate that BthTX-I, BthTX-II and PrTX-I recruit leucocyte into the pleural cavity of the rat by mechanisms unrelated to enzymatic activity and pleural mast cell degranulation. |
| Databáze: | OpenAIRE |
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