Effective method for synthetic peptide immobilization that increases the sensitivity and specificity of ELISA procedures
| Autor: | A. T. Kozhich, Z. K. Suvorova, Vadim T. Ivanov, Vadim S. Ivanov, Leonid D. Tchikin |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Chemistry Immunology Molecular Sequence Data Human immunodeficiency virus (HIV) Peptide Enzyme-Linked Immunosorbent Assay Elisa assay HIV Antibodies medicine.disease_cause Molecular biology Peptide antigen Antigen HIV Seropositivity medicine biology.protein Immunology and Allergy Humans Amino Acid Sequence Antibody |
| Zdroj: | Journal of immunological methods. 153(1-2) |
| ISSN: | 0022-1759 |
| Popis: | A procedure is described for the immobilization of synthetic peptide antigens on a plastic solid phase for performing ELISA. The use of a streptavidin-biotinylated peptide system for coating microplates with peptide antigen markedly increased both the sensitivity and the specificity compared to a standard ELISA based on synthetic peptides. the procedure was used for the detection of HIV-1-specific antibodies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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