Inexpensive protein overexpression driven by the NarL transcription activator protein

Autor: Joanne Hothersall, Sandie Lai, Nan Zhang, Rita E. Godfrey, Patcharawarin Ruanto, Sarah Bischoff, Colin Robinson, Tim W. Overton, Stephen J. W. Busby, Douglas F. Browning
Jazyk: angličtina
Rok vydání: 2022
Předmět:
ISSN: 0006-3592
1097-0290
Popis: Most Escherichia coli overexpression vectors used for recombinant protein production (RPP) depend on organic inducers, for example, sugars or simple conjugates. However, these can be expensive and, sometimes, chemically unstable. To simplify this and to cut the cost of RPP, we have developed vectors controlled by the Escherichia coli nitrate-responsive NarL transcription activator protein, which use nitrate, a cheap, stable, and abundant inorganic ion, to induce high-level controlled RPP. We show that target proteins, such as green fluorescent protein, human growth hormone, and single-chain variable region antibody fragments can be expressed to high levels using our promoter systems. As nitrate levels are high in many commercial fertilizers, we demonstrate that controlled RPP can be achieved using readily available and inexpensive garden products.
Databáze: OpenAIRE
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