A Feasibility Study for Diagnosis of Latent Tuberculosis Infection Using an IGRA Point-of-Care Platform in South Korea
| Autor: | Hazel M. Dockrell, Dong Hwan Choi, Ji Young Hong, Kyungjoon Kang, Jeong Min Lee, Sang Nae Cho, Hoodon Joo, Yun Gyoung Hur, So Yeong Park, Ahreum Kim, Yoonsuk Lee, Min-Suk Kwon |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male medicine.medical_specialty Tuberculosis Point-of-Care Systems Positive control Infectious Disease Enzyme-Linked Immunosorbent Assay 030204 cardiovascular system & hematology Words: ichroma™ IGRA-TB Positive correlation Mycobacterium tuberculosis 03 medical and health sciences Interferon-gamma Young Adult 0302 clinical medicine Latent Tuberculosis Active tb Internal medicine Republic of Korea medicine latent TB infection Humans IFN-γ Point of care biology Latent tuberculosis Receiver operating characteristic business.industry QuantiFERON-TB Gold In-Tube test Tuberculin Test point-of-care platform General Medicine medicine.disease biology.organism_classification tuberculosis ROC Curve 030220 oncology & carcinogenesis Area Under Curve Case-Control Studies Feasibility Studies Original Article Female Reagent Kits Diagnostic business Interferon-gamma Release Tests |
| Zdroj: | Yonsei Medical Journal |
| ISSN: | 1976-2437 0513-5796 |
| Popis: | PURPOSE: This study aimed to evaluate ichroma™ IGRA-TB, a novel point-of-care platform for assaying IFN-γ release, and to compare it with QuantiFERON-TB Gold In-Tube (QFT-GIT) for identifying Mycobacterium tuberculosis (M. tb) infection. MATERIALS AND METHODS: We recruited 60 healthy subjects, and blood samples were obtained in QFT-GIT blood collection tubes. The blood collection tubes were incubated at 37°C, and culture supernatant was harvested after 18-24 hours. IFN-γ responses were assessed by the ichroma™ IGRA-TB cartridge and the QFT-GIT IFN-γ enzyme-linked immunosorbent assay. Three active TB patients were recruited as a positive control for M. tb infection. RESULTS: The area under the receiver operating characteristic curve of the ichroma™ IGRA-TB test for differentiating between infected and non-infected individuals was 0.9706 (p |
| Databáze: | OpenAIRE |
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