Stability-indicating RP-HPLC method for the determination of 6-gingerol in polyherbal formulations
| Autor: | Sayeed Ahmad, Y.T. Kamal, Shahana Salam, Mhaveer Singh, Prawez Alam |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
education.field_of_study
6-gingerol Chromatography Population General Physics and Astronomy General Chemistry Pharmacology High-performance liquid chromatography General Biochemistry Genetics and Molecular Biology Linear relationship Linear regression Stability indicating Forced degradation General Materials Science Stress conditions education General Environmental Science Mathematics |
| Zdroj: | Journal of Analytical Science and Technology. 6(1) |
| ISSN: | 2093-3371 |
| DOI: | 10.1186/s40543-015-0056-3 |
| Popis: | Background Among different systems medicine practiced worldwide, Unani medicine is the classical one and still commonly practiced in India and abroad for centuries. As it is widely used by a majority of population, it is necessary to come up with a systematic approach to develop well-designed methodologies for the quality control of different polyherbal formulations which are used for treatment of various diseases in this system of medicine. Methods A reverse phase stability indicating HPLC method was developed for the determination of 6-gingerol in polyherbal formulations. Separation of 6-gingerol was achieved on reverse phase C18 (250 × 4.6 mm) column with a mobile phase containing methanol: 0.05% orthophosphoric acid in water (60:40, v/v) at 280 nm using UV-visible detector. The flow rate was kept as 1 mL/min. The proposed method was validated according to ICH guidelines for accuracy, precision, robustness, LOD, and LOQ, and statistical analysis proved method was accurate, precise, and robust. Results The linear regression analysis data showed a good linear relationship (r 2 = 0.9989 ± 0.0010) for 6-gingerol in the concentration range of 0.5 μg to 500 μg/mL. This proved the method can be employed for the determination 6-gingerol even in nanogram levels. The detection (LOD) and quantification (LOQ) limits were found to be 0.05 μg and 0.18 μg/mL, respectively. Satisfactory recovery results were observed from the herbal compound formulations (98.5 to 101%). Intra- and inter-day precisions of the method were found convincing, with relative standard deviation (%RSD) values in the range of 0.5 to 1.9%. Forced degradation studies of 6-gingerol was also carried out under different stress conditions which showed that the drug is stable in acidic and basic conditions whereas unstable against dry heat. Conclusions Hence, newly validated HPLC method can be used for the quality control and standardization of different herbal formulations which contain Zingiber officianalis as one of the ingredients. |
| Databáze: | OpenAIRE |
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