Long Noncoding RNA LINC01125 Enhances Cisplatin Sensitivity of Ovarian Cancer via miR-1972
| Autor: | Hua Pan, Jia Guo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Apoptosis
Carcinoma Ovarian Epithelial 030204 cardiovascular system & hematology 03 medical and health sciences 0302 clinical medicine Cell Movement Lab/In Vitro Research Cell Line Tumor medicine Humans Cytotoxicity Cell Proliferation Ovarian Neoplasms Cisplatin Regulation of gene expression Chemistry Cell growth RNA General Medicine medicine.disease Long non-coding RNA Gene Expression Regulation Neoplastic MicroRNAs Drug Resistance Neoplasm Cell culture 030220 oncology & carcinogenesis Cancer research Female RNA Long Noncoding Ovarian cancer Signal Transduction medicine.drug |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| Popis: | BACKGROUND Ovarian cancer (OC) is the most frequent aggressive cancer among women worldwide, and chemoresistance is the major challenge in the clinical treatment of OC. Recently, there is evidence that long noncoding RNAs (lncRNAs) are closely related to the regulation of cisplatin (CDDP) resistance in OC cells. However, whether LINC01125, a novel lncRNA, can improve the sensitivity of OC to cisplatin remains unknown. MATERIAL AND METHODS In this study, we analyzed aberrantly expressed lncRNAs in miR-200a-overexpressing OC samples by using GSE122123. LINC01125 and miR-1972 expressions were measured by qRT-PCR. The effect of LINC01125 overexpression on cell proliferation was determined by CCK-8 and colony formation assays. The sensitivity of OC cells to cisplatin was determined by CCK-8 assays. The interaction between LINC01125 and miR-1972 was verified through dual-luciferase reporter and RNA immunoprecipitation (RIP) assays, and bioinformatics analysis was performed to predict the target genes of miR-1972. RESULTS Our results indicated that LINC01125 expression was significantly downregulated in CDDP-resistant OC tissues and cell lines. Overexpression of LINC01125 inhibited OC cell proliferation and enhanced the cytotoxicity of CDDP in OC cells. Additionally, LINC01125 participated in the apoptosis pathway by directly binding to miR-1972 in OC cells. CONCLUSIONS Therefore, we suggest that LINC01125 might act as a tumor suppressor in OC and enhances the cisplatin sensitivity of OC cells by binding to miR-1972. |
| Databáze: | OpenAIRE |
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