Apc-Mutant Kyoto Apc Delta (KAD) Rats Are Susceptible to 4-NQO-Induced Tongue Carcinogenesis
| Autor: | Naoki Watanabe, Yohei Shirakami, Takafumi Naiki, Takuji Tanaka, Tadao Serikawa, Hisataka Moriwaki, Takashi Kuramoto, Kazuto Yoshimi, Takayuki Mori, Takahiro Kochi, Masahito Shimizu |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Tongue squamous cell carcinoma Mutant Inflammation medicine.disease_cause lcsh:RC254-282 Article susceptibility Proinflammatory cytokine Tongue tongue Internal medicine Apc mutant rats medicine business.industry lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.anatomical_structure Endocrinology Oncology inflammation Immunohistochemistry Cancer development medicine.symptom Carcinogenesis business carcinogenesis |
| Zdroj: | Cancers Volume 6 Issue 3 Pages 1522-1539 Cancers, Vol 6, Iss 3, Pp 1522-1539 (2014) |
| ISSN: | 2072-6694 |
| Popis: | Despite widening interest in the possible association between infection/ inflammation and cancer development, knowledge of this issue in relation to oral cancer remains inadequate. This study aimed to determine the susceptibility of Apc-mutant Kyoto Apc Delta (KAD) rats, which are vulnerable to developing inflammation-associated colorectal carcinogenesis, to 4-nitroquinoline 1-oxide (4-NQO)-induced tongue carcinogenesis in order to clarify the role of inflammation in oral cancer. KAD (20 males and 22 females) and F344/NS1c (22 males and 23 females) rats received drinking water with or without 4-NQO (20 ppm) for eight weeks. Histopathological and immunohistochemical analyses of the tongue were performed at week 20. Additionally, the mRNA expression of inflammatory cytokines in the tongue mucosa was determined at week 8. Tongue squamous cell carcinoma (SCC) developed in the KAD and F344/NS1c rats that received 4-NQO. Regardless of gender, the incidence and multiplicity of tongue SCC were greater in the KAD rats than in the F344/NS1c rats. In addition, the multiplicity of tongue SCC in the female KAD rats was significantly greater than that observed in the male KAD (p < 0.01) and female F344/NS1c rats (p < 0.05). The levels of inflammation and the mRNA expression of inflammatory cytokines in the tongue in the 4-NQO-treated female KAD rats were the highest among the rats given 4-NQO. These results show that KAD rats, particularly females, are susceptible to 4-NQO-induced tongue carcinogenesis, suggesting the utility of models employing KAD rats for investigating the pathobiology of oral (tongue) carcinogenesis associated with inflammation. |
| Databáze: | OpenAIRE |
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