Improving Mycoplasma ovipneumoniae culture medium by a comparative transcriptome method
| Autor: | Xiaohui Wang, Wen-guang Zhang, Yongqing Hao |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
culture medium
040301 veterinary sciences Biology Microbiology 0403 veterinary science Serine Transcriptome 03 medical and health sciences Mycoplasma ovipneumoniae Nucleotide 030304 developmental biology chemistry.chemical_classification Bacteriological Techniques 0303 health sciences General Veterinary Strain (chemistry) Nucleotides 04 agricultural and veterinary sciences Metabolism biology.organism_classification Culture Media Biochemistry chemistry Cell culture Original Article Mycoplasma mycoides |
| Zdroj: | Journal of Veterinary Science |
| ISSN: | 1976-555X 1229-845X |
| DOI: | 10.4142/jvs.2020.21.e30 |
| Popis: | Mycoplasma ovipneumoniae (Mo) is difficult to culture, resulting in many difficulties in related research and application. Since nucleotide metabolism is a basic metabolism affects growth, this study conducted a “point-to-point” comparison of the corresponding growth phases between the Mo NM151 strain and the Mycoplasma mycoides subsp. capri (Mmc) PG3 strain. The results showed that the largest difference in nucleotide metabolism was found in the stationary phase. Nucleotide synthesis in PG3 was mostly de novo, while nucleotide synthesis in NM151 was primarily based on salvage synthesis. Compared with PG3, the missing reactions of NM151 referred to the synthesis of deoxythymine monophosphate. We proposed and validated a culture medium with added serine to fill this gap and prolong the stationary phase of NM151. This solved the problem of the fast death of Mo, which is significant for related research and application. |
| Databáze: | OpenAIRE |
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