Molecular-level dengue fever diagnostic devices made out of paper
| Autor: | Jiann-Hwa Chen, Shih-Jie Lo, Da-Jeng Yao, Wu-Chun Tu, Shih-Chun Yang, Chao-Min Cheng |
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| Rok vydání: | 2013 |
| Předmět: |
Paper
Biomedical Engineering Bioengineering Dengue virus Biology medicine.disease_cause Sensitivity and Specificity Biochemistry Dengue fever Dengue chemistry.chemical_compound Molecular level Viral genetics Image Processing Computer-Assisted medicine Humans Dna viral Fluorescent Dyes Reverse Transcriptase Polymerase Chain Reaction Equipment Design General Chemistry Nucleic acid amplification technique Dengue Virus medicine.disease Virology Molecular Diagnostic Techniques chemistry DNA Viral RNA Viral Oligonucleotide Probes Nucleic Acid Amplification Techniques DNA Nucleic acid detection |
| Zdroj: | Lab on a Chip. 13:2686 |
| ISSN: | 1473-0189 1473-0197 |
| Popis: | This paper describes the development of nucleic acid detection in paper using a combination of commercial fluorescent probes and DNA ladders, and provides us with a better understanding of the interactions between double-stranded DNA (the amplified products in this study), fiber structures in paper, and fluorescent probes. The amplified products (the reverse-transcription and amplification of dengue virus serotype-2 RNA via RT-LAMP) in this study were subsequently fluorescently labeled in paper-based test zones (on our paper-based diagnostic device), thus fluorescent probes were used to perform the diagnosis of dengue fever, specific to serotype-2. |
| Databáze: | OpenAIRE |
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