Selenium‐sensitive miRNA‐181a‐5p targeting SBP2 regulates selenoproteins expression in cartilage
| Autor: | Mengyao Sun, Yuanxu Guo, Yitong Zhao, Yan Han, Huang Huang, Jian Sun, Dongxian Guo, Zixin Min, Qilan Ning, Peng Xu, Lisong Heng, Nannan Zhong, Shemin Lu, Safdar Hussain, Fujun Zhang |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
GPX1 SBP2 Anabolism Down-Regulation miRNA‐181a‐5p selenoprotein GPX4 Models Biological Cell Line 03 medical and health sciences chemistry.chemical_compound Selenium Chondrocytes Animals Humans Selenoproteins chemistry.chemical_classification Reporter gene 030102 biochemistry & molecular biology Selenocysteine Base Sequence Catabolism RNA-Binding Proteins Cell Biology Original Articles Chondrogenesis Cell biology Diet Rats MicroRNAs 030104 developmental biology Cartilage chemistry Molecular Medicine Original Article Selenoprotein Signal Transduction |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| Popis: | Selenium (Se) deficiency brings about defects in the biosynthesis of several selenoproteins and has been associated with aberrant chondrogenesis. Selenocysteine (Sec) Insertion Sequence (SECIS) and SECIS binding protein 2 (SBP2) interaction is a very critical node for the metabolic balance between Se and selenoproteins. The Gpx1, Gpx4 and SelS have different binding affinities with SBP2 in cells. According to our results, both miR‐181a‐5p and SBP2 appeared to be selenium‐sensitive and regulated the expression of selenoproteins in C28/I2 cells under Se sufficient environment. However, they showed significantly opposite expression trend in Se deficiency rats cartilage and SeD C28/I2 cells. The SBP2 is a direct target gene of miR‐181a‐5p in C28/I2 cells as determined by reporter gene and off‐target experiments. And the miR‐181a‐5p could regulate SBP2 and the selenoproteins in C28/I2 cells. Depending upon the Se supply levels, C28/I2 cells were divided into three groups, that is normal Se, SeD and SeS, which underwent through a 7‐day Se deprivation process, then SBP2 was knocked‐down and overexpressed in all the groups. Moreover, the selected selenoproteins were down‐regulated in second‐generation low Se diet rat cartilage. The selenoproteins expression was decreased by Se deficiency which depended on the Selenium‐sensitive miR‐181a‐5p to participate and regulate SBP2 at post‐transcriptional level. It involves a series of antioxidant and ECM (extracellular matrix) genes, to overcome the ROS‐related stress for the protection of essential physiological functions and to maintain the balance between anabolism and catabolism of the cartilage. |
| Databáze: | OpenAIRE |
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