Effects of freezing, fixation and dehydration on surface roughness properties of porcine left anterior descending coronary arteries
| Autor: | Daniel M. Espino, Hanna E. Burton, Richard L. Williams |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
Tissue Fixation Surface Properties Swine 0206 medical engineering General Physics and Astronomy 02 engineering and technology Specimen Handling Fixation (surgical) Structural Biology Freezing Image Processing Computer-Assisted medicine Surface roughness Animals General Materials Science Dehydration Desiccation Chemistry Optical Imaging Cell Biology Anatomy 021001 nanoscience & nanotechnology medicine.disease Coronary Vessels 020601 biomedical engineering Surgery Coronary arteries medicine.anatomical_structure 0210 nano-technology |
| Zdroj: | Micron. 101:78-86 |
| ISSN: | 0968-4328 |
| DOI: | 10.1016/j.micron.2017.06.009 |
| Popis: | To allow measurements of surface roughness to be made of coronary arteries using various imaging techniques, chemical processing, such as fixation and dehydration, is commonly used. Standard protocols suggest storing fresh biological tissue at -40°C. The aim of this study was to quantify the changes caused by freezing and chemical processing to the surface roughness measurements of coronary arteries, and to determine whether correction factors are needed for surface roughness measurements of coronary arteries following chemical processes typically used before imaging these arteries.Porcine left anterior descending coronary arteries were dissected ex vivo. Surface roughness was then calculated following three-dimensional reconstruction of surface images obtained using an optical microscope. Surface roughness was measured before and after a freeze cycle to assess changes during freezing, after chemical fixation, and again after dehydration, to determine changes during these steps of chemical processing.No significant difference was caused due to the freeze cycle (p0.05). There was no significant difference in the longitudinally measured surface roughness (RaIndependently, freezing, fixation and dehydration do not alter the surface roughness of coronary arteries. Combined, however, fixation and dehydration significantly increase the circumferential, but not longitudinal, surface roughness of coronary arteries. |
| Databáze: | OpenAIRE |
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