Molecular characterisation and risk factor analysis of Cryptosporidium spp. in calves from Italy
Autor: | Pablo Díaz, Pablo Díez-Baños, Anna Paola Pipia, A. Scala, A. Ruiu, Antonio Varcasia, P. Spissu, G Sanna, Claudia Tamponi, Alberto Prieto, Patrocinio Morrondo |
---|---|
Rok vydání: | 2018 |
Předmět: |
gp60 subtyping
Male 0301 basic medicine Veterinary medicine Farms Cryptosporidium infection animal diseases 030231 tropical medicine Cryptosporidium Cattle Diseases Cryptosporidiosis Weaning Feces 03 medical and health sciences 0302 clinical medicine Risk Factors parasitic diseases Genotype Prevalence medicine Pre-weaned calves Animals Risk factor Original Paper General Veterinary biology Oocysts General Medicine DNA Protozoan 030108 mycology & parasitology biology.organism_classification medicine.disease Infectious Diseases Cryptosporidium parvum Italy Parasitology RNA Ribosomal SSU rRNA genotyping Insect Science Herd Cattle Female |
Zdroj: | Parasitology Research |
ISSN: | 1432-1955 0932-0113 |
Popis: | To provide up-to-date information on the occurrence of Cryptosporidium in pre-weaned calves from Sardinia (Italy), the species implicated and their zoonotic potential, 147 faecal samples from 22 cattle herds were microscopically examined for Cryptosporidium oocysts; positive isolates were molecularly characterised. A questionnaire was developed to identify risk factors for Cryptosporidium infection. Overall, the percentage of positive calves and farms was 38.8 and 68.2%, respectively. The SSU rRNA-based PCR identified two Cryptosporidium species, Cryptosporidium parvum (95.8%) and C. bovis (4.2%). Sequence analyses of the glycoprotein (gp60) gene revealed that all C. parvum isolates belonged to the subtype family IIa (IIaA15G2R1 and IIaA16G3R1), with the exception of three isolates that belonged to the subtype family IId (IIdA20G1b and IIdA20). Mixed logistic regression results indicated that calves aged 15–21 days were more likely to be Cryptosporidium-positive. The risk of being positive was also significantly higher in herds from Central Sardinia and in farms using non-slatted flooring. In addition, the application of disinfectants and milk replacers was significantly associated with higher Cryptosporidium prevalence. In contrast, the risk of being positive was significantly reduced in halofuginone-treated calves. Our results reveal that a significant percentage of suckling calves are carriers of zoonotic subtypes of C. parvum. Thus, both healthy and diarrhoeic calves younger than 1 month may represent a risk for the transmission of cryptosporidiosis in humans and animals. Electronic supplementary material The online version of this article (10.1007/s00436-018-6000-x) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
Externí odkaz: |