A Highly Effective Protocol for the Rapid and Consistent Induction of Digital Dermatitis in Holstein Calves

Autor: Paul J. Plummer, Patrick J. Gorden, Vickie L. Cooper, Adam C. Krull, John W. Coatney, Jan K. Shearer
Rok vydání: 2016
Předmět:
0301 basic medicine
Pathology
Biopsy
Lameness
Animal
Antibiotics
lcsh:Medicine
Pure Culture
Beef cattle
Pathology and Laboratory Medicine
0403 veterinary science
Medicine and Health Sciences
lcsh:Science
Musculoskeletal System
Multidisciplinary
Treponema
biology
medicine.diagnostic_test
Antimicrobials
Drugs
04 agricultural and veterinary sciences
Infectious Diseases
Veterinary Diseases
Lameness
Legs
Digital Dermatitis
Biological Cultures
Anatomy
Research Article
Cell Culturing Techniques
DNA
Bacterial
medicine.medical_specialty
Histology
Infectious Disease Control
040301 veterinary sciences
medicine.drug_class
Cattle Diseases
Surgical and Invasive Medical Procedures
Research and Analysis Methods
Microbiology
03 medical and health sciences
Signs and Symptoms
Diagnostic Medicine
Microbial Control
medicine
Animals
Dairy cattle
Pharmacology
business.industry
lcsh:R
Limbs (Anatomy)
Digital dermatitis
Biology and Life Sciences
medicine.disease
biology.organism_classification
Disease Models
Animal
030104 developmental biology
Lesions
lcsh:Q
Veterinary Science
Cattle
Feet (Anatomy)
business
Zdroj: PLoS ONE
PLoS ONE, Vol 11, Iss 4, p e0154481 (2016)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0154481
Popis: Bovine Digital Dermatitis (DD) is a leading cause of lameness in dairy cattle. DD is reportedly increasing in prevalence in beef cattle feedlots of the US. The exact etiologic agent(s) responsible for the disease have yet to be determined. Multiple studies have demonstrated the presence of a variety of Treponema spp. within lesions. Attempts to reproduce clinically relevant disease using pure cultures of these organisms has failed to result in lesions that mirror the morphology and severity of naturally occurring lesions. This manuscript details the systematic development of an experimental protocol that reliably induces digital dermatitis lesions on a large enough scale to allow experimental evaluation of treatment and prevention measures. In total, 21 protocols from five experiments were evaluated on their effectiveness in inducing DD lesions in 126 Holstein calves (504 feet). The protocols varied in the type and concentration of inoculum, frequency of inoculation, duration the feet were wrapped, and type of experimental controls need to validate a successful induction. Knowledge gained in the first four experiments resulted in a final protocol capable of inducing DD lesions in 42 of 44 (95%) feet over a 28 day period. All induced lesions were macroscopically and microscopically identified as clinical DD lesions by individuals blinded to protocols. Lesions were also located at the site of inoculation in the palmer aspect of the interdigital space, and induced clinically measurable lameness in a significant portion of the calves. Collectively these results validate the model and provide a rapid and reliable means of inducing DD in large groups of calves.
Databáze: OpenAIRE
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